Biomarkers of Meats and Potatoes Intake

Biomarkers of Meats and Potatoes Intake: a Meal Study in Healthy Men and Women - the MEPO Study

Status

Completed

Phases

Study type

Interventional

Source

ClinicalTrials.gov

Registry ID

NCT04548362

Acronym

MEPO

Enrollment

Registered

2020-09-14

Start date

2016-02-15

Completion date

2016-05-30

Last updated

2021-04-28

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Nutrition Disorders

Keywords

Biomarkers of food intake

Brief summary

This cross-over meal study is a four-way intervention with 12 volunteers randomized at the same time to four meals with fried meats (beef, pork, chicken and amino-acid matched non-meat control) and to four meals with potato products (boiled, chips, fries, and a control with boiled white rice) to search for biomarkers of intake.

Detailed description

In a two-dimensional, cross-over meal study, 12 healthy volunteers consumed in a randomized sequence four test meals: chicken, pork, beef, and a control made of egg white and pea. At the same time they were independently randomized to four starchy foods to be consumed together with the meats: boiled potatoes, fried potatoes, potato crisps or boiled white rice. Fasting and postprandial urine samples were collected to cover 48 h after each meal and samples were profiled by untargeted LC-ESI-qTOF-MS metabolomics. The profiles following the meal challenges were explored by univariate and multivariate analyses and single and combined markers of intake identified chemically by MS/MS fragmentation experiments and statistically by ROC curves and error rates as well as FDR statistics.

Interventions

OTHERMeat arm

Randomised sequence of pork, beef, chicken meat, and an egg white/pea combination

OTHERStarchy foods arm

Randomised sequence of boiled potato, fried potato, potato crisps, and boiled white rice

Study design

Allocation

RANDOMIZED

Intervention model

FACTORIAL

Primary purpose

BASIC_SCIENCE

Masking

NONE

Masking description

It is not possible to mask foods and for the explorative analysis of results, masking is not required.

Intervention model description

Subjects were randomised to four meals with meats and starchy foods in such a way that all volunteers had each meat and each starchy food once and so that all combinations of meats and starchy foods were equally common. Each volunteer was also randomised to the sequence of the meals.

Eligibility

Sex/Gender

ALL

Age

18 Years to 70 Years

Healthy volunteers

Yes

Inclusion criteria

* healthy

Exclusion criteria

* non-pregnant, no abuse of alcohol or drugs, no chronic medication except p-pills

Design outcomes

Primary

Measure	Time frame	Description
meat intake biomarkers	0-48 hours	To identify intake biomarkers of general meat, red meat and poultry
Potato intake biomarkers	0-48 hours	To identify intake biomarkers of general potato, fried potato and white rice

Countries

Denmark

Outcome results

None listed

Source: ClinicalTrials.gov · Data processed: Feb 4, 2026