Importance of the Microtubule Cytoskeleton in Oocyte Competence

ART

Oocyte competence, Oocyte vitrification, Cryobiology, Microtubule cytoskeleton

This study will assess if there are microtubule cytoplasmic features specifically associated with oocyte vitrification.

Successful fertilization is heavily dependent upon inherent qualities of the oocytes, and thus reliant upon the fidelity of oocyte maturation. Reduced oocyte developmental competence is one of the main reasons for the decreased potential of in-vitro-produced embryos. Approximately 8,6% to 15% of all infertility patients produce at least one meiotically incompetent oocyte. There are also extreme cases of women which generate oocytes with complete or predominant failure to complete meiosis. These oocytes arrest and do not complete meiosis in response to exogenous final maturation and ovulation triggering. The incidence of this is unknown, and given the complexity of the processes involved in oocyte maturation, it has been difficult to define the key events or morphological features underlying oocyte competence. An increasing amount of evidence in the past years has suggested that the microtubule (MT) cytoskeleton may have an important role in providing the oocyte with competence potential. Oocyte maturation involves major rearrangements of the MT cytoskeleton. It is well known the importance of forming a proper microtubule meiotic spindle for chromosome segregation upon meiosis. Moreover, MTs seem to be important for the redistribution of different organelles upon maturation. However, the exact functional significance of this reorganization and how MTs influence competence through the repositioning of these organelles is largely unknown. The main MT organizing center in eukaryotic cells is the centrosome. Indeed, centrosomes are essential for the formation of the mitotic MT bipolar spindle. Interestingly, in the vast majority of female eggs these structures are eliminated, and the meiotic spindle does not contain centrosomes. A recent study in the lab organism Drosophila melanogaster (fruit fly), showed that interrupting this elimination, forcing the maintenance of centrosomes up to meiosis, led to female infertility. Both meiosis and the first nuclear divisions upon fertilization showed abnormal MT spindles with abnormal chromosome congression. This shows that proper spaciotemporal MT organization is likely to have important implications on oocyte competence. The long-term goal of this research group is to analyze the MT cytoskeleton of oocytes from women which, upon assisted reproductive technologies, generate a high percentage of arrested embryos. In order to do this, the MT features of already-available surplus and unused oocytes which are vitrified and available for research purposes will be compared. These oocytes were donated from cycles in which a low (\<20% the fertilized embryos) and high (\>20% the fertilized embryos) incidence of arrested embryos was seen. Previous studies have posited that oocyte vitrification and warming may expose the oocyte to a variety of physical and chemical processes which may impact on their structural and genomic integrity. Therefore, this initial feasibility pilot study will investigate potential structural consequences for the oocytes of the vitrification procedure used in house. Thus, it will be assess whether the Cryotop® - Open System induces specific MT features which may be absent in fresh oocytes. In case that both fresh and vitrified oocytes (derived from the same patient - sibling oocytes study) show a similar MT cytoskeleton organization, a collection of cryopreserved oocytes donated for research purposes will be used in the planned future studies of this research group.

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