Sleep Deprivation
Conditions
Brief summary
Insufficient sleep is associated with an increased risk for cardiovascular disease. The causal mechanisms are currently unknown, but may include endothelial dysfunction. The purpose of this study is to examine the influence of sex and aging on the effects of total sleep deprivation on vascular function and whether exercise training attenuates these effects.
Interventions
OTHERExercise
60 minutes of moderate-intensity treadmill exercise (70% of maximal heart rate determined from the maximal graded exercise test)
OTHERSupervised Total Sleep Deprivation
Laboratory-monitored total sleep deprivation
Sponsors
University of Florida
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
BASIC_SCIENCE
Masking
NONE
Eligibility
Sex/Gender
ALL
Age
20 Years to 60 Years
Healthy volunteers
Yes
Inclusion criteria
* Males and females * 20 to 30 and 50 to 60 years of age * Females will be eumenorrheic at enrollment or postmenopausal for at least 1 year * No major clinical disease (e.g., diabetes, cardiovascular, liver or chronic kidney disease) to minimize confounding of vascular measures * Participants will be sedentary (≤3x per week of ≤30 minutes of aerobic exercise/session) or trained (≥5x per week of moderate/vigorous aerobic exercise training)
Exclusion criteria
* Age \<20 or 31 to 49 or \>60 years * Body mass index ≥30 kg/m\^2 because obesity may affect vascular function * Use of medication that may affect vascular measures * Hormone replacement therapy or hormonal contraceptives within past year * Use of tobacco products (chewing tobacco, traditional or e-cigarettes) because they may influence vascular function * Being perimenopausal, pregnant or lactating because may influence vascular function * Being a shift worker because habitual sleep deprivation and altered circadian rhythm may influence vascular function. * Not having an intermediate chronotype based on the Morningness-Eveningness Questionnaire (MEQ; score \<31 or \>69) because this would influence the effect of overnight sleep deprivation on vascular function. * Sleep complaints based on the Pittsburgh Sleep Quality Index (PSQI; score \>5) because this would influence the effect of overnight sleep deprivation on vascular function.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Endothelial function | through study completion, an average of 1 month | Endothelial function will be determined using brachial artery flow-mediated dilation via high resolution duplex ultrasonography. Reactive hyperemia will be produced by inflating a forearm cuff to \ 250 mmHg for 5 minutes followed by rapid deflation. |
| Microvascular function | through study completion, an average of 1 month | Microvascular function will be assessed using the forearm blood flow response to submaximal handgrip exercise. Brachial artery blood flow and diameter will be assessed using Doppler ultrasound. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Central arterial stiffness | through study completion, an average of 1 month | The SphygmoCor XCEL device will be used to measure carotid to femoral pulse wave velocity. |
| Central arterial hemodynamics | through study completion, an average of 1 month | The SphygmoCor XCEL device will be used to obtain central pulse wave analysis. |
Countries
United States
Outcome results
None listed