Hepatic Glycogen
Conditions
Brief summary
The purpose of this study is to investigate the effect of prolonged overnight fasting in the hepatic glycogen stores
Interventions
BEHAVIORALOvernight fasting duration
Subjects will adhere to overnight fasting protocol for 1 day
Sponsors
Maastricht University
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
BASIC_SCIENCE
Masking
NONE
Eligibility
Sex/Gender
ALL
Age
45 Years to 75 Years
Healthy volunteers
Yes
Inclusion criteria
* Participants are able to provide signed and dated written informed consent prior to any study specific procedures * Caucasian (people will be excluded when having a ≥50% racial African/Asian background) * Participants should have suitable veins for cannulation or repeated venipuncture * Women are post-menopausal (defined as at least 1 year post cessation of menses) * Men and women aged ≥ 45 and ≤ 75 years at the start of the study * Body mass index (BMI) 18.5 - 25 kg/m2 * Stable dietary habits (no weight loss or gain \>3kg in the past 3 months) * Sedentary lifestyle (not more than 3 hours of vigorous sports per week)
Exclusion criteria
* Type 2 Diabetes Mellitus * Active diseases (cardiovascular, diabetes, liver, kidney, cancer or other) * Any contraindication for MRI scanning * Alcohol consumption of \>2 servings per day * Regular smoking * No use of medication interfering with investigated study parameters (as determined by responsible physician) * Participants who do not want to be informed about unexpected medical findings, or do not wish that their physician
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Hepatic glycogen content | 1 hour | Measured with 13C-MRS |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Substrate oxidation | 14 hours | Measured as RQ |
Other
| Measure | Time frame | Description |
|---|---|---|
| Hepatic fat oxidation | 5 hours | measured as plasma BHB levels |
| Liver volume | 5 minutes | Measured with MRI |
| Intrahepatic fat content and composition (1H-MRS) | 30 minutes | Measured with 1H-MRS |
| Plasma metabolites related to energy metabolism | 5 hours | measured in plasma samples |
| Body composition | 5 minutes | fat mass/fat free mass measured with BodPod |
| Substrate oxidation | 30 minutes | measured with indirect calorimetry |
| De novo lipogenesis (DNL) | 20 hours | measured as percentage of palmitate in VLDL-TG originating from DNL |
Countries
Netherlands
Outcome results
None listed