Immune System Diseases, Lymphocytic Infiltrate, Pancreatitis
Conditions
Keywords
pancreatitis, lymphocyte, Flow-cytometry
Brief summary
Pancreatitis is a common complication especially in patients with gallbladder stones, most patients with biliary pancreatitis may recover spontaneously without sequelae, but in 10-20% of patients, the disease is severe and mortality rates of up to 30% are detected in these patients. In the evaluation of acute biliary pancreatitis, many scoring systems have been established (Atlanta, Ranson, APACHE, BISAP etc.) from past to present to determine morbidity and mortality of the disease. In this study, the investigators aimed to evaluate the correlation between morbidity and mortality of acute biliary pancreatitis and lymphocyte subtypes with Flow-cytometry.
Detailed description
Pancreatitis is a common complication especially in patients with gallbladder stones, most patients with biliary pancreatitis may recover spontaneously without sequelae, but in 10-20% of patients, the disease is severe and mortality rates of up to 30% are detected in these patients. In patients with severe pancreatitis, aggressive fluid replacement, organ damage follow-up, appropriate antibiotherapy, and endoscopic sphincterotomy and radiological interventions may be of great benefit. In the evaluation of acute biliary pancreatitis, many scoring systems have been established (Atlanta, Ranson, APACHE, BISAP etc.) from past to present to determine morbidity and mortality of the disease. There are limited number of studies in the literature about the immune parameters in the evaluation of acute pancreatitis. In a studies, serum inflammatory markers such as IL-1, IL-6 and CD4, CD8 T lymphocyte and Treg population were evaluated.Treg cells are reported to be an independent prognostic factor in determining the severity of acute pancreatitis. In patients diagnosed with acute biliary pancreatitis, determination of the course of the disease at the time of diagnosis is extremely important for treatment and survival. In this study, the investigators aimed to evaluate the correlation between morbidity and mortality of acute biliary pancreatitis with lymphocyte subtypes via Flow-cytometry.
Interventions
DIAGNOSTIC_TESTFlow Cytometric analysis
Blood samples will be taken from the patients with biliary pancreatitis and healthy volunteers to perform flow-cytometric analysis and to determine lymphocyte subgroups
Sponsors
Istanbul Training and Research Hospital
Study design
Observational model
CASE_CONTROL
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
18 Years to 80 Years
Healthy volunteers
Yes
Inclusion criteria
* 18-80 years old * Diagnosis of acute biliary pancreatitis * Sign the voluntary consent form
Exclusion criteria
* Being under 18 or older than 80 * Not signing the voluntary consent form * Pancreatitis resulting from an interventional procedure (ERCP, surgery, etc.) * Pregnant women * Being a history of immunodeficiency * Cancer history
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Lymphocyte subtypes 1 | 9 months | T helper (CD4) (%) |
| Lymphocyte subtypes 2 | 9 months | T cytotoxic (CD8) (%) |
| Lymphocyte subtypes 3 | 9 months | Treg (CD25 and CD127) (%) |
Countries
Turkey (Türkiye)
Outcome results
None listed