Rotator Cuff Tears, Rotator Cuff Tendinosis
Conditions
Keywords
ultrasonography, rotator cuff tendinopathy
Brief summary
The purpose of this project is to extract effusions and damaged tendons during tendon repair therapy in patients with tendon tears. Then, we would analyze tendon-related transcription factors from histology and cytology, and compare changes in tendon with ultrasound images. Helps to understand the mechanism of tendon lesions.
Detailed description
According to past studies, it is often observed that changes in extracellular matrix, growth factors, and cytokines in degenerative tendons, indicating that the tendon is constantly changing. This change will in turn produce a change in the morphology of the tendon tissue in the ultrasound image. However, there have been few studies in the past literature on the correlation between constant changes in tendon-specific transcription factors and characterization on clinical ultrasound images. Therefore, the purpose of this project is to extract the effusion and damaged tendon while performing tendon repair therapy in patients with tendon tears, and to analyze the tendon-related transcription factors from histology and cytology to understand the changes in tendon homeostasis. At the same time, the interpretation of clinical ultrasound images was combined to establish the relationship between the biochemical factors of the cell tissues of chronic tendon lesions and the clinical ultrasound image. This study will help us to understand the mechanism of chronic tendinopathy.
Interventions
None listed
Sponsors
National Taiwan University Hospital
Study design
Observational model
OTHER
Time perspective
CROSS_SECTIONAL
Eligibility
Sex/Gender
ALL
Age
20 Years to No maximum
Healthy volunteers
No
Inclusion criteria
* age older than 20 y * shoulder pain more than 3 months and Visual Analogue Scale (VAS) \>4 * shoulder tendon defect on ultrasound images
Exclusion criteria
* diagnosis of cervical radiculopathy or other central or peripheral neuropathy * rheumatoid arthritis or other autoimmune diseases * have received any shoulder joint injection within three months * tumor or systematic inflammation
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| correlation between ultrasound image and tendon tissue sectioning and staining | Month 2 | comparing the image of ultrasound and tendon tissue sectioning and staining to understand the tendon changing in micro stage while the defect could be singed on the ultrasound image |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| tendon-related transcription factors | Month 1 | Tendon tissue sectioning and staining Immunohistochemical staining DNA microarray Tendon cell culture Flow cytometry Real-time quantitative reverse transcription polymerase chain reaction RNA-interference-mediated gene silencing |
Countries
Taiwan
Contacts
Primary ContactChao Yuan-Hung, PhD
Outcome results
None listed