Inflammation, Oxidative Stress
Conditions
Brief summary
A comparison of acute tart cherry formations (juice vs. powdered) and doses (single vs. twice daily) on inflammation and oxidative capacity.
Detailed description
Participants will be randomly allocated to a group: one tart cherry capsule daily, two tart cherry capsules daily, one 8oz. bottle of tart cherry juice daily, two 8oz. bottles of tart cherry juice taken 8 hours apart, one placebo pill, and one 8oz. bottle of placebo. Participants will arrive to the lab at 8 am after an overnight fast of 10 hours. They will provide a blood sample and then ingest their study treatment. Participants will stay in the lab for a further 2 hours for additional blood draws then return to the lab in 6 hours for another blood draw. At this time if they are in a twice daily group they will receive their second treatment for the day. All participants will come back to the lab 24 hours later where they will give a blood sample and receive their next treatment. If they are in the twice daily group they will be provided with their next dose and given instructions to consume it in 8 hours. Participants will arrive 24 hours later for a final blood draw with no supplementation. Blood samples will be measured pre-ingestion, 1 hour, 2 hours, 8 hours, 24 hours and 48 hours post-ingestion. Samples will be analyzed for plasma oxygen radical absorbance capacity (ORAC), uric acid (UA), and C-reactive protein (CRP).
Interventions
DIETARY_SUPPLEMENTtart cherry
Either tart cherry juice or freeze dried powdered tart cherry in capsule was given
DIETARY_SUPPLEMENTplacebo
either cornstarch capsule or kool-aid
Sponsors
Marywood University
Ohio University
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
BASIC_SCIENCE
Masking
DOUBLE (Subject, Investigator)
Eligibility
Sex/Gender
ALL
Age
18 Years to 45 Years
Healthy volunteers
Yes
Inclusion criteria
* free of cardiovascular, metabolic and inflammatory conditions * non-smokers * no known allergy to cherries or cherry juice * not be taking food supplements (turmeric/curcumin, cherry products, greens, etc.).
Exclusion criteria
* quit smoking less than one year ago. * currently being treated for arthritis or an inflammatory condition. * currently being treated for uncontrolled cardiovascular disease, high blood pressure, diabetes, fibromyalgia, or irritable bowel syndrome. * pregnant. * regularly consume cherries or are allergic to cherries or dairy. * currently use anti-inflammatory medications, * have used corticosteroids in the last two months.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Change in c-reactive protein | 1 hour, 2 hours, 8 hours, 24 hours, and 48 hours post ingestion | c-reactive protein (mg/L) measured via assay |
| Change in uric acid | 1 hour, 2 hours, 8 hours, 24 hours, and 48 hours post ingestion | uric acid (mg/dL) measured via assay |
| Change in oxidative capacity | 1 hour, 2 hours, 8 hours, 24 hours, and 48 hours post ingestion | oxygen radical absorbance capacity (uM Trolox equivalents) measured via assay |
Outcome results
None listed