Dietary Exposure, Metabolic Disturbance
Conditions
Brief summary
With free sugar intake proving to be of a concern within the general public, discovery and validation of a new biomarker will allow for more consistent measurement of sucrose intake. Furthermore, using a multi-omic approach the investigators will identify metabolic perturbations to the metabolome and proteome.
Detailed description
Excessive free sugar intake is of concern within the general public, as intakes have been associated with weight gain and cardiovascular disease. Average intakes are over double that of the 5% of total energy intake that is recommended by the Science Advisory Committee on Nutrition, but intakes are calculated from observational measures that lack sensitivity and discovery and validation of a new biomarker from biological fluids may allow for more specific measurement and a better understanding of intake:disease relationships. Furthermore, understanding the biochemistry of sucrose intake will allow the identification of damage occurrence and alternative metabolic pathways, as well as novel protein damage that occur with chronic sucrose exposure. This study aims to identify a biomarker of chronic sucrose consumption using metabolite profiling technology. The study will be composed of a randomised controlled intervention trial, in which participants will be required to consume an amount of sucrose (0-120g/d) every day for 7 days and provide biofluid samples (urine and blood) before the initiation, during and following the intervention; that will undergo metabolic analysis. Furthermore, participants will have their anthropometrics and dietary intake monitored throughout the study. The biomarker will also be validated against the dietary information and correlated with indices of health and sucrose-induced damage. The investigators will also monitor the feasibility and acceptability of chronic sucrose intake during the intervention.
Interventions
DIETARY_SUPPLEMENTsucrose
pure sucrose in water in addition to habitual diet
Sponsors
Northumbria University
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
BASIC_SCIENCE
Masking
SINGLE (Subject)
Intervention model description
RCT
Eligibility
Sex/Gender
ALL
Age
19 Years to 64 Years
Healthy volunteers
Yes
Inclusion criteria
* Age 19-64, healthy diagnosis
Exclusion criteria
* gastrointestinal issues, doesn't consume sugar
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Blood pressure | change in blood pressure following one week intervention | Measurement of systolic and diastolic blood pressure |
| Metabolome | change in the urinary metabolome following one week intervention | Metabolite profiling of urine samples, obtaining a profile of approximately 5000 metabolite intensities in urine |
| Body composition | change in body mass index following one week intervention | measurement of body mass index |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| food diary analysis | change in dietary intake following the week intervention | measurement of energy, macro and micronutrient intakes from 3-day food diaries |
Countries
United Kingdom
Outcome results
None listed