Aneuploidy Rate, Infertility, Mosaicism
Conditions
Keywords
PGT-A, Mosaicism, Single step media, Sequential media
Brief summary
A prospective cohort study undergoing ICSI at a private fertility center in Egypt
Detailed description
Purpose: To evaluate PGTai results for embryos cultured in sequential versus single step media. Method: Injected oocytes will divided between Continuous Single Culture-NX media (CSC- NX) with 10% SSS the first group (Irvine, USA) and the other group will be cultured in sequential cleavage and blastocyst media with 10% SPS (SAGE, Denmark). All blastocytes with 4BB quality and better according to Gardner's criteria 1999 will go through PGTA procedures with PGTai confirmation.
Interventions
OTHERSingle step media
Usage of single step media in embryo culture
OTHERSequential media
Usage of sequential media in embryo culture
Sponsors
Ganin Fertility Center
Study design
Allocation
NON_RANDOMIZED
Intervention model
PARALLEL
Primary purpose
OTHER
Masking
NONE
Intervention model description
Evaluating the embryonic development and PGTai results for embryos cultured in sequential media versus single step media.
Eligibility
Sex/Gender
ALL
Age
18 Years to 37 Years
Healthy volunteers
No
Inclusion criteria
* Female age ≤37 years with ≥ 8 mature oocytes * Male partner had normal semen parameters according to WHO 2010 * PGT-A was done at the blastocyst stage
Exclusion criteria
* sperm or oocyte donor * severe male factors according to WHO 2010
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Euploidy rate | 5:7 days of intracytoplasmic sperm injection(ICSI) | percentage of euploid embryos/ total genetic tested embryos on blast stage |
| Mosaicism rate | 5:7 days of ICSI | percentage of mosaic embryos/ total genetic tested embryos on blast stage |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| High quality of cleaved embryos | 72 hours of ICSI | High quality D3 embryos according to Gardner's criteria / total cleaved embryos |
| Fertilization rate | 16:19 hours of ICSI | Percentage of fertilized oocyte (appearance of two pronuclei) / Number of injected oocytes |
| High quality blast rate | 5:7 days of ICSI | Morphological grading of trophectoderm and Inner cell mass using Gardner's criteria 1999, from grade A and B ( A is the highest quality). Total high grade embryos/ total blast embryos. |
| Blast formation rate | 5:7 days of ICSI | Percentage of total blastocysts / Total cleaved embryos |
| Cleavage stage | 72 hours of ICSI | Percentage of cleaved embryos/ total number of fertilized oocytes |
Countries
Egypt
Contacts
Primary ContactManar M Hozyen, MSc.
Backup ContactAmr Elshimy, BSc.
Outcome results
None listed