Culture Media Microdroplet Geometry With Different Oil Overlay and Its Effect on Embryonic Development.

The Effect of Culture Media Microdroplet Geometry and Different Oil Overlay on Pre Implantation Embryonic Development in Dry Incubators: Prospective Randomized Study.

Status

Completed

Phases

Study type

Interventional

Source

ClinicalTrials.gov

Registry ID

NCT04485806

Enrollment

100

Registered

2020-07-24

Start date

2019-08-01

Completion date

2020-01-10

Last updated

2020-07-24

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Embryonic Development, Infertility

Keywords

dry incubator, Osmolality, Oil overlay, Microdrops

Brief summary

A prospective randomized study included 1695 MII sibling oocytes collected from 100 patients undergoing ICSI.

Detailed description

Purpose: to determine the effect of culture media microdroplet geometry of different culture dishes and oil overlay on the pre implantation embryo development in dry incubators Method: We used sibling oocytes splits between two different dishes : GPS (life global, USA) and SPL (life science, Korea) with two different oils : light mineral oil (LM)(Irvine, USA) and paraffin oil (PO)(Vitro life, Sweden). All embryos cultured in dry incubators at the same conditions. statistical analysis done using SPSS version 23

Interventions

OTHERGPS/LM

Media microdroplet geometry (3D dishes) in combination with light mineral oil overlay

OTHERSPL/LM

Media microdroplet geometry (2D dishes) in combination with light mineral oil overlay

OTHERGPS/PO

Media microdroplet geometry (3D dishes) in combination with Paraffin oil overlay

OTHERSPL/PO

Media microdroplet geometry (2D dishes) in combination with paraffin oil overlay

Study design

Allocation

RANDOMIZED

Intervention model

PARALLEL

Primary purpose

OTHER

Masking

NONE

Intervention model description

Effect of two different culture dishes with two different oil overlay which used in human embryos culturing through ICSI procedures on the pre implantaion embryonic development.

Eligibility

Sex/Gender

FEMALE

Age

18 Years to 37 Years

Healthy volunteers

Inclusion criteria

* Female age is ≤ 37 years old with ≥ 8 MII oocytes * Males with normal semen parameters (WHO 2010). * We conducted three experiments all with Siblings oocytes splits and all embryos

Exclusion criteria

* Oocyte or sperm donation. * Sever male factor. * Any contradictions to undergoing in vitro fertilization or goandotropin stimulation.

Design outcomes

Primary

Measure	Time frame	Description
Blastulation rate	5-6 days after ICSI	Percentage of total blastocysts / Total cleaved embryos
High quality blast rate	5-6 days after ICSI	Morphological grading of trophectoderm and Inner cell mass using Gardner's criteria 1999, from grade A and B ( A is the highest quality). Total high grade embryos/ total blast embryos.

Secondary

Measure	Time frame	Description
Fertilization rate	16-19 hours of ICSI	Percentage of fertilized oocyte (appearance of two pronuclei) / Number of injected oocytes
Cleavage rate	72 hours of ICSI	Percentage of cleaved embryos/ total number of fertilized oocytes
High quality D3 embryos	72 hours of ICSI	High quality D3 embryos according to Gardner's criteria / total cleaved embryos
Good quality trophectoderm/inner cell mass	5-6 days after ICSI	better grade than 3BB of blast embryos according to Gardner's criteria 1999

Countries

Egypt

Outcome results

None listed

Source: ClinicalTrials.gov · Data processed: Feb 4, 2026