Meningioma
Conditions
Brief summary
In this study, meningioma organoids will be established from patients' tissues and the characterization of the organoid will be performed.
Detailed description
Aims and Hypothesis to be tested: The investigators hypothesized that the derived organoid should have a similar morphological and genetic mutation signatures to the patient primary tissue. Study period: 1 year for patient recruitment and organoid generation from patient tumor Study population: 30 Study samples: Patient's meningioma tumor sample are collected from patients with surgical needs. Sample collected are used for organoid culture. Morphological and genetic characteristics will be compared between the patient tumor sample and the corresponding derived organoids Primary outcome measures: Whole exome sequencing data of patient tumor tissue and patients-derived organoids
Interventions
None listed
Sponsors
Chinese University of Hong Kong
Study design
Observational model
OTHER
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
18 Years to No maximum
Healthy volunteers
No
Inclusion criteria
1. Adult patients (aged \>18 years) with meningioma 2. Admission into Prince Wales hospitals neurosurgical services 3. Patients with surgical needs of meningioma removal
Exclusion criteria
1\. Patients (or next-of-kin as appropriate) refuse to participate into the study
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Whole exome sequencing data | 7 days | DNA extracted from patient tumor tissue and patients-derived organoids will be perform whole exome sequencing for genetic characterization. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Immunostaining | 7 days | H&E staining and meningioma markers immunostaining |
Countries
Hong Kong
Outcome results
None listed