Optimizing Platelet Isolation (The Cell Study)

Optimizing Platelet Isolation for Measures of Human Platelet Mitochondrial Respiration (The Cell Study)

Status

Completed

Phases

Unknown

Study type

Observational

Source

ClinicalTrials.gov

Registry ID

NCT04470505

Enrollment

Registered

2020-07-14

Start date

2020-07-06

Completion date

2021-01-25

Last updated

2025-10-17

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Healthy

Brief summary

The research study is about blood cells (platelets) and their power houses (mitochondria). The investigators want to learn about their behavior in order to ensure that our laboratory procedures are generating optimal results.

Detailed description

The objectives of this study are to assess platelet activation following standard laboratory procedures used in the process of measuring platelet mitochondrial function, and to determine the optimal amount of digitonin needed to permeabilize platelet cell membrane.

Interventions

None listed

Study design

Observational model

COHORT

Time perspective

PROSPECTIVE

Eligibility

Sex/Gender

ALL

Age

25 Years to 35 Years

Healthy volunteers

Yes

Inclusion criteria

* Age 25-35 * All races * All ethnicity * 18.5 - 30 BMI

Exclusion criteria

* smoking * Aspirin intake * Recreational drug use * Alcohol intake more than 3 times per week * Self-reported possibility of current pregnancy * Known diagnosis of: * Pre-diabetes * Diabetes * High cholesterol * High triglycerides * Hypertension * Anemia * Autism spectrum disorder * Attention deficit hyperactivity disorder (ADHD) * Attention deficit disorder (ADD) * Severe asthma * Refusal to stop taking nutritional supplements * Any other pre-existing medical condition that requires daily medication as determined by the investigators to affect outcome of interest

Design outcomes

Primary

Measure	Time frame	Description
Platelet activation from isolated platelets	day 1	Whole blood will be collected following an overnight fast, and platelets isolated using standard procedures. Platelet activation markers (e.g., CD62P ) will be measured using flow cytometric analysis.

Secondary

Measure	Time frame	Description
Platelet activation measured in whole blood	day 1	Whole blood will be collected following an overnight fast. Platelet activation will be measured in whole blood by the expression CD62P
Platelet reactivity measured in whole blood	day 1	Whole blood will be collected following an overnight fast. Platelet reactivity will be measured in whole blood with adenosine diphosphate (ADP).

Countries

United States

Outcome results