T Cell Deficiency
Conditions
Brief summary
The primary end-point of our prospective, observational study is to count T cells in patients with laboratory-confirmed COVID-19 and healthy controls. In addition, the expression of T cell exhaustion marker was measured in COVID-19 cases.
Detailed description
COVID-19 caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has posed great threat to human health. T cells play a critical role in antiviral immunity but their numbers and functional state in COVID-19 patients remain largely unclear. The immune response against viral infections depends on the activation of cytotoxic T cells that can clear infection by killing virus-infected cells, so boosting the numbers and function of T cells in COVID-19 patients is critical for successful recovery. However, the factors which might cause the reduction in count, and the activation status of T cells in COVID-19 patients, remain uninvestigated. Thus demonstration of T cell exhaustion during COVID-19 infection suggest that more urgent, early intervention may be required in patients with low T lymphocyte counts.
Interventions
DIAGNOSTIC_TESTFlow cytometry
Lymphocyte subsets in peripheral blood were assessed by staining 50 µl of blood sample with 5 µl of Fluoroisothiocyanate (FITC)-conjugated- PD-1, phycoerythrin (PE)-conjugated-CD8, peridinium-chlorophyll-protein (Per-CP)-conjugated-CD4, Peridinium-chlorophyll-protein (Per-CP)-conjugated anti-CD3 and allophycocyanin (APC) conjugated anti-CD28.
Sponsors
Assiut University
Study design
Observational model
CASE_CONTROL
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Healthy volunteers
Yes
Inclusion criteria
* Diagnosis of Covid-19 test positive; hospitalized subjects; both sexes; given informed consent.
Exclusion criteria
* no
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| CD4+ (T-helper cells) in COVID 19 patients and healthy volunteers. | Baseline | Detection of the percentage of CD4+ (T-helper cells) in COVID 19 patients and healthy volunteers. |
| CD8+ (T-cytotoxic cells) in COVID 19 patients and healthy volunteers. | Baseline | Detection of the percentage of CD8+ (T-cytotoxic cells) in COVID 19 patients and healthy volunteers . |
| Detect PD-1 on CD8+ and CD4+ cells in COVID 19 patients and healthy volunteers . | Baseline | Detection of the percentage of expression of PD-1 on CD8+ and CD4+ cells in COVID 19 patient and healthy volunteers. |
| Detect CD28+ and CD3+ cells in COVID 19 patients and healthy volunteers. | Baseline | Detection of the frequency of CD28+ and CD3+ cells in COVID 19 patients and healthy volunteers . |
Countries
Egypt
Contacts
Primary ContactHebatallah Hassan, Lecturer
Outcome results
None listed