Healthy
Conditions
Keywords
Staphylococcus aureus, ICAM-1, nasal cells, S. aureus, nasal swab
Brief summary
An inflammatory state of the nasal cells (very close to keratinocytes) could favour the internalization of Staphylococcus aureus and thus constitute a persistent reservoir for the carriage of this bacterium. Staphylococcus aureus is a commensal bacterium of the skin and mucous membranes that colonizes approximately 2 billion people worldwide Staphylococcus aureus is also a leading cause of community and healthcare-associated infection. Staphylococcus aureus has demonstrated its ability to invade many non-professional phagocytic cell lines such as keratinocytes, osteoblasts, fibroblasts, epithelial cells and endothelial cells. During pro-inflammatory stimulation, internalization of Staphylococcus aureus into keratinocytes is mainly mediated by ICAM-1. These results suggest that, in humans, an inflammatory state of the nasal cells (very close to keratinocytes) could promote the internalization of Staphylococcus aureus and thus constitute a persistent reservoir for the carriage of this bacterium.
Detailed description
The main objective is to compare the expression level of ICAM-1 in nasal cells with and without the presence of Staphylococcus aureus.
Interventions
DIAGNOSTIC_TESTnasal swab
nasal swab will be performed to analysis biological markers at every visit.
Sponsors
Centre Hospitalier Universitaire de Saint Etienne
Study design
Allocation
NA
Intervention model
SINGLE_GROUP
Primary purpose
DIAGNOSTIC
Masking
NONE
Eligibility
Sex/Gender
ALL
Age
18 Years to No maximum
Healthy volunteers
Yes
Inclusion criteria
* Subject affiliated to a social security scheme or entitled person * Subject who received informed information about the study et signed consent * Adult subject (≥18ans) * Healthy Volunteer
Exclusion criteria
* Subject undergoing antibiotic treatment or having received antibiotic treatment (oral, IV or local) in the month prior to inclusion * Subject with a major haemostasis disorder or anticoagulant treatment contraindicated for surgery * Subject with a pathology increasing the nasal carriage of S. aureus (chronic furunculosis, diabetes, HIV infection, chronic rhinosinusitis) * Subject under guardianship or trusteeship * Pregnant woman
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| level of ICAM-1 expression in nasal cells | Months: 0 | Compare the level of ICAM-1 expression in nasal cells with and without the presence of intracellular Staphylococcus aureus |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Measurement of the expression of the genes (mRNA dosage) involved in IL-1 beta secretion | Months: 0 | Measure by rt-qPCR technique. |
| Measurement of the expression of the genes (mRNA dosage) involved in activation of autophagy | Months: 0 | Measure by rt-qPCR technique. |
| cytokine concentration | Months: 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12 | Measure by analysis of nasal swab. |
| concentration of ICAM-1 in S. Aureus | Months: 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12 | Measure by analysis of nasal swab. |
Countries
France
Outcome results
None listed