Investigation of Supragingival Plaque Microbiome in Early Childhood Caries

Investigation of Supragingival Plaque Microbiome Using Next Generation Sequencing in Early Childhood Caries

Status

Completed

Phases

Unknown

Study type

Observational

Source

ClinicalTrials.gov

Registry ID

NCT04456335

Enrollment

Registered

2020-07-02

Start date

2019-03-04

Completion date

2020-05-23

Last updated

2020-07-02

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Early Childhood Caries

Keywords

Early childhood caries, Oral Microbiome, Next generation sequencing

Brief summary

The purpose of this study was to characterize the supragingival plaque microbiome in children with early childhood caries and identify potential microorganisms associated with caries.

Detailed description

Sixty children with and without early childhood caries were recruited from Trakya University Faculty of Dentistry Department of Pediatric Dentistry. Oral examinations were performed by the same examiner according to diagnostic criteria of World Health Organisation (WHO) and The International Caries Detection and Assessment System (ICDAS) . Mothers' completed a structured questionnaire on the socio-demographic informations, child's feeding practices and oral hygiene habits. After the oral examination, supragingival plaque samples were collected from the posterior tooth surfaces. Supragingival plaque samples were examined with the Illumina MiSeq new generation sequencing method.

Interventions

GENETICCollection of supragingival plaque from caries free children

Supragingival plaque samples were collected from posterior molar teeth

Study design

Observational model

CASE_CONTROL

Time perspective

OTHER

Eligibility

Sex/Gender

ALL

Age

36 Months to 71 Months

Inclusion criteria

* 36-71 months old children * No history of any medical problem * No history of antibiotic in the three months prior to sampling * Any sign of early childhood caries, * Caries free children * Mothers accepted to complete a structured questionnaire * Children who cooperate for dental examination and sample collection

Exclusion criteria

* Children older than 71 months * Medically compromised * Children with antibiotic use history at last three months * Children refused dental examination and plaque collection * Mothers' refused to complete questionnare

Design outcomes

Primary

Measure	Time frame	Description
V4-16S rRNA and ITS1 rRNA sequencing	6 months	Supragingival plaque samples were collected from the tooth surfaces using a sterile periodontal curette. DNA extraction was performed at Sugenomik Biotechnology Laboratory for library preparation and paired-end Illumina MiSeq PE250 sequencing of the V4 region of bacterial 16S rRNA and fungal ITS1 (internal transcribed spacer 1) rRNA genes.

Countries

Turkey (Türkiye)

Outcome results

None listed

Source: ClinicalTrials.gov · Data processed: Feb 4, 2026