Laser Assistance to Improve ICSI Outcome
Conditions
Keywords
Laser assisted-ICSI, Zona pellucida thinning, ICSI, embryo good quality rate, hatching rate
Brief summary
laser-assisted ICSI (LA-ICSI) was described to be a new method combining a less invasive ICSI technique with assisted hatching.This procedure is usually used for MII oocytes possessing oolemma breakage difficulties, but why not using it for even normal Oocytes?!. Especially because data indicated that in addition to an improved oocyte survival, this new approach increases the hatching rate in vitro. But since it is still debatable that such a small opening might impair the hatching process, so modified zona thinning technique was proposed for more embryo safety.
Detailed description
This study aims to compare the LA- ICSI by drilling a micro hole (LAD-ICSI) and modified LA-ICSI by zona pellucida thinning (LAT-ICSI) with conventional ICSI in order to effectively assess whether this approach can be applied routinely to improve ICSI outcome. One arm study divided into three interventions; Conventional ICSI (control group) was compared with LAD-ICSI (Study group 1) and LAT- ICSI (study group 2) in sibling oocytes. The rates of maturation, fertilization, Devision, Blastulation and good quality blastulation rates were assessed in the three groups Cases were randomly selected for embryo transfer from the three groups,Then chemical pregnancy and clinical pregnancy rates were compared between the three groups.
Interventions
PROCEDURELaser Assisted ICSI
using OCTICS laser device to do small opening or zona thinning to improve ICSI outcome
Sponsors
Royal Fertility Center, Egypt
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
TREATMENT
Masking
DOUBLE (Subject, Caregiver)
Intervention model description
study divided into three interventions; Conventional ICSI (control group) was compared with LAD-ICSI (Study group 1) and LAT- ICSI (study group 2) in sibling oocytes. then Cases randomly selected for embryo transfer from each group
Eligibility
Sex/Gender
FEMALE
Age
20 Years to 37 Years
Healthy volunteers
Yes
Inclusion criteria
* Patients aged from 20 years old to 37 years old * good res-ponders * gives more than 15 oocytes * endometrial thickness more than 9 mm
Exclusion criteria
* poor res-ponders * severe oligoasthinozoospermia of male partners * testicular samples * Poor quality oocytes
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Clinical pregnancy rate | three-four weeks after embryo transfer | confirmed by sac observation using ultrasound |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Blastulation rate | 5 days later after ICSI procedure | number of blastocysts that are grown from devided embryos |
| Hatching rate | 5 days later after ICSI procedure | number of blastocysts that hatch out of zona |
Countries
Egypt
Outcome results
None listed