Exercise Training, Physical Activity
Conditions
Keywords
Exercise, Physiology
Brief summary
Exercise training is beneficial for both health and performance. Histamine has been shown to be involved in the acute exercise response. The current study addresses the role of histamine H1/H2 receptor signaling in the chronic training-induced adaptations. Results from this study will yield more insights into the molecular mechanisms of adaptations to exercise training.
Interventions
OTHERLactose
Placebo: Lactose capsules
H1 receptor antagonist: 540 mg Fexofenadine Hydrochloride
DRUGFamotidine
H2 receptor antagonist: 40 mg Famotidine
OTHERHigh-intensity interval training (HIIT)
6 weeks HIIT
Sponsors
Research Foundation Flanders
University of Copenhagen
University Ghent
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
BASIC_SCIENCE
Masking
QUADRUPLE (Subject, Caregiver, Investigator, Outcomes Assessor)
Masking description
Double-blind for researchers and participants
Intervention model description
Placebo + exercise vs histamine blockade + exercise
Eligibility
Sex/Gender
MALE
Age
18 Years to 50 Years
Healthy volunteers
Yes
Inclusion criteria
* Sedentary or low levels of physical activity * Caucasian
Exclusion criteria
* Chronic diseases * Medication use * Smoking * Excessive alcohol consumption * Seasonal allergies
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Change in cardiorespiratory fitness | Before, after 3 weeks and after 6 weeks of exercise training | Change in maximal oxygen uptake during incremental cycling test on cycle ergometer during the 6 week training period |
| Change in peak aerobic power output | Before, after 3 weeks and after 6 weeks of exercise training | Change in peak power output during incremental cycling test on cycle ergometer during the 6 week training period |
| Change in whole-body insulin sensitivity | Before and after 6 weeks of exercise training | Change from baseline in Matsuda index for whole-body insulin sensitivity derived from Oral Glucose Tolerance Test after the 6 week training period |
| Change in microvascular function | Before and after 6 weeks of exercise training | Change from baseline in microvascular function (Single Passive Leg Movement technique) after the 6 week training period |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Change in skeletal muscle capillarization | Before and after 6 weeks of exercise training | Change from baseline in skeletal muscle capillarization (immunohistochemistry) after the 6 week training period |
| Change in skeletal muscle enzyme activity | Before and after 6 weeks of exercise training | Change from baseline in enzyme activity assessment of markers of relevance for skeletal muscle function after the 6 week training period |
| Change in skeletal muscle protein content | Before and after 6 weeks of exercise training | Change from baseline in Western Blot assessment of markers of relevance for skeletal muscle function after the 6 week training period |
| Change in power output at Gas Exchange Threshold (GET) | Before, after 3 weeks and after 6 weeks of exercise training | Change from baseline in GET during incremental cycling test after the 6 week training period |
| Change in power output at Respiratory Compensation Point (RCP) | Before, after 3 weeks and after 6 weeks of exercise training | Change from baseline in RCP during incremental cycling test after the 6 week training period |
| Change in time to exhaustion performance test | Before, after 3 weeks and after 6 weeks of exercise training | Change in time to exhaustion test (performed after incremental cycling test) during the 6 week training period |
| Change in heart rate during submaximal cycling | Before and after 6 weeks of exercise training | Change from baseline in heart rate during submaximal cycling after the 6 week training period |
| Change in substrate oxidation during submaximal cycling | Before and after 6 weeks of exercise training | Change from baseline in substrate oxidation during submaximal cycling test (estimated via gas exchange data) after the 6 week training period |
| Change in blood lactate accumulation during submaximal cycling | Before and after 6 weeks of exercise training | Change from baseline in capillary lactate concentration at end of submaximal cycling test after the 6 week training period |
| Change in cycling efficiency during submaximal cycling | Before and after 6 weeks of exercise training | Change from baseline in cycling efficiency (estimated via gas exchange data) after the 6 week training period |
| Change in fasted serum insulin concentrations | Before and after 6 weeks of exercise training | Change from baseline in fasted blood concentrations of insulin after the 6 week training period |
| Change in fasted serum glucose concentrations | Before and after 6 weeks of exercise training | Change from baseline in fasted blood concentrations of glucose after the 6 week training period |
| Change in fasted serum cholesterol concentrations | Before and after 6 weeks of exercise training | Change from baseline in fasted blood concentrations of cholesterol after the 6 week training period |
| Change in fasted serum triglyceride concentrations | Before and after 6 weeks of exercise training | Change from baseline in fasted blood concentrations of triglyceride after the 6 week training period |
| Change in resting blood pressure | Before and after 6 weeks of exercise training | Change from baseline in resting mean arterial blood pressure after the 6 week training period |
| Change in resting heart rate | Before and after 6 weeks of exercise training | Change from baseline in resting heart rate after the 6 week training period |
| Change in body weight | Before and after 6 weeks of exercise training | Change from baseline in total body weight after the 6 week training period |
Countries
Belgium
Outcome results
None listed