Hand Eczema
Conditions
Brief summary
This project aims to investigate the prevalence of bacterial colonization and associated factors in health care workers with hand eczema with focus on Staphylococcus aureus.
Detailed description
Two groups will be examined in this study: health care workers with HE and matched controls invited through a questionnaire study which will be sent to approximately 4000 health care workers in Capital Region. Samples from health care workers with and without hand eczema will be collected after obtaining their written informed consent. Collection of samples: Swabs will be rubbed on the skin for 30 seconds on lesional and non-lesional skin on the dominating hand and from the nostrils. The swabs will immediately be stored in -80 C° (research biobank) for subsequent analysis at Statens Serum Institute.
Interventions
OTHEReSwabs
from skin
Sponsors
Statens Serum Institut
University Hospital Bispebjerg and Frederiksberg
Study design
Observational model
CASE_CONTROL
Time perspective
CROSS_SECTIONAL
Eligibility
Sex/Gender
ALL
Age
18 Years to 70 Years
Healthy volunteers
Yes
Inclusion criteria
* The diagnosis of hand eczema (for group with hand eczema)
Exclusion criteria
* Other skin diseases (in both groups)
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Staphyloccous aureus presence | Baseline, cross-sectional | Prevalence of S. aureus colonization on hands and in nose in two groups of health care workers. The swab samples will be assessed by culturing on selective S. aureus plates (chromID S. aureus; bioMerieux, Marcy l'Etoile, France) and incubated overnight at 37 °C. DNA will be purified from S. aureus isolates using the Qiagen DNeasy Blood and Tissue Purification Kit (Qiagen, Hilden, Germany). |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| CC-types and spa-typing | Baseline, cross-sectional | clonal complex (CC) type and spa-types are characterizing the S. aureus. Will be correlated to departments and professions of health care workers. The S. aureus will be further characterized by spa typing, which will be performed by polymerase chain reaction and Sanger sequencing of the product. The respective CC types will be assigned from the spa type using eBURST (http://eburst.mlst.net). |
| Hand Eczema Severity Index (HECSI) | Baseline, cross-sectional | Validated severity measurement tool for hand eczema performed by the investigator. Severity will be correlated to S. aureus colonization and CC types |
Countries
Denmark
Contacts
Primary ContactYasemin T. Yüksel, MD
Backup ContactTove Agner, MD, PhD
Outcome results
None listed