Sepsis, Immunologic Paralysis, Immunologic Suppression, Immune Deficiency
Conditions
Keywords
sepsis, septic shock, immune response, immunoparalysis, transcriptomics, flow-cytometry, immunomodulation
Brief summary
Detailed description of immune response and its dynamics in sepsis and septic shock patiens by means of transcriptomics, flow-cytometry and cytokine analysis.
Interventions
OTHERtranscriptomics
transcriptomics
OTHERflow-cytometry
peripheral blood myeloid cells and lymphocytes flow-cytometry
OTHERcytokines
peripheral blood cytokine analysis
Sponsors
Charles University, Czech Republic
Study design
Observational model
CASE_CONTROL
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
18 Years to No maximum
Healthy volunteers
Yes
Inclusion criteria
* sepsis/septic shock (SEPSIS-3) * age ≥18 * informed consent * inclusion within first 24hrs after development/diagnosis of septic shock
Exclusion criteria
* disagreement of the patient or legal representative with the entry into the study * patients with primary or secondary immunodeficiency * presence of active haematological malignancy or an active non-haematological malignancy
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Immune response description - flow cytometry | 2024 - 2026 | Detailed description of immune response analyzed by mean of flow cytometry |
| Immune response description - transcriptomics | 2024 - 2026 | Detailed description of immune response analyzed by mean of transcriptomic analysis |
| Immune response description - ELISA | 2024 - 2026 | Detailed description of immune response analyzed by mean of ELISA |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Diagnostic immune status panel | 2026 - 2028 | Diagnostic tool for immune status assessment |
Countries
Czechia
Contacts
Primary ContactThomas Karvunidis, MD, Ph.D.
Backup ContactMarcela Královcová, MD
Outcome results
None listed