Characteristics of Abdominal Fluid in Patients With Diverticulitis Hinchey III or IV

Study of Changes in Abdominal Fluid in Connection With Peritonitis. A Descriptive Study in Patients With Purulent or Fecal Peritonitis.

Status

Active, not recruiting

Phases

Unknown

Study type

Observational

Source

ClinicalTrials.gov

Registry ID

NCT04427891

Enrollment

Registered

2020-06-11

Start date

2021-09-21

Completion date

2027-12-01

Last updated

2026-04-27

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Perforated Diverticulitis

Keywords

perforated diverticulitis, peritonitis, protein changes, microbiota

Brief summary

Perforated diverticulitis of the colon can be complicated by peritonitis, either purulent or fecal. Both are life threatening conditions requiring emergency surgery. The background in terms of reasons for inflammation (diverticulitis) and perforation to develop is unknown. In this study we focus on specific changes associated with inflammation, both in tissues and of fecal and mucosal microbiota.

Detailed description

A prospective study of abdominal fluid, periferal blood, tissues as well as feces in Patients (perforated diverticulitis with peritonitis undergoing emergency surgery, Group I), and in Controls (patients operated due to colorectal cancer, Group II; patients with mild diverticulitis not needing surgery, Group III). Immune acitvity in abdominal fluid and periferal blood by immunoassay of 92 individual proteins. mRNA expression of 96 specific genes in tissue samples. Quatification of bacterial diversity in feces and colon mucosa.

Interventions

None listed

Study design

Observational model

OTHER

Time perspective

PROSPECTIVE

Eligibility

Sex/Gender

ALL

Healthy volunteers

Inclusion criteria

* Signs of perforated diverticulitis with peritonitis and decision to perform emergency surgery (Group I) * Signs of acute diverticulitis and no decision of emergency surgery (Group II) * Patients with colorectal cancer planned for elective surgery (Group III)

Exclusion criteria

* No surgery performed (Group I and III) * Withdrawn consent (any group)

Design outcomes

Primary

Measure	Time frame	Description
inflammatory response	index surgery (Groups I and II)/ emergency room (Group III)	determination of levels of 92 proteins in abdominal fluid and blood

Secondary

Measure	Time frame	Description
inflammatory response in tissue	index surgery (Groups I and II)/ emergency room (Group III)	RNA sequencing (96 spcific genes) in abdominal tissue
microbiota composition	index surgery (Groups I and II)/ emergency room (Group III)	PCR amplification, cloning and sequencing of genes

Countries

Sweden

Contacts

PRINCIPAL_INVESTIGATOREva Haglind, MD,PhD

Sahlgrenska univesitetssjukhuset

Outcome results