Muscle Disuse
Conditions
Brief summary
This is an interventional study enrolling healthy individuals aged 18-35 and 60-85 to understand the recovery of muscle health following a period of inactivity. The enrollment goal is 45 participants. The study will occur over the course of 1-2 months where participants will undergo testing before and after a 2-week limb immobilization period.
Detailed description
Visit 1 (Pre-testing)- After signing a consent form, participants will undergo a blood screening and an oral glucose tolerance test. After the visit, participants will be provided with a step activity monitor to track their level of activity. A dietary assessment will also be determined. Visit 2- At a later time, the participant will return to the research center for a biopsy, blood draw, body composition scan, MRI and muscle strength testing. 2-week leg immobilization period- After visit 2 , the participant will return home and undergo a 14-day leg immobilization period using the equipment and instructions provided at visit 2. Visit 3- During the limb immobilization period, participant will return to the research center for a single blood draw. Visit 4- At the end of the 2-week limb immobilization period, the participant will return to the research center to undergo another biopsy, blood draw, body composition scan, MRI and muscle strength testing. Visit 5- 2 days after the 2-week limb immobilization period, the participant will return for a biopsy and blood draw. Visit 6- 7 days after the 2-week limb immobilization period, the participant will be asked to return for a final biopsy, blood draw, body composition scan, MRI and muscle strength testing.
Interventions
OTHERLimb immobilization
Participants will undergo a 2-week period of leg immobilization
Sponsors
University of Utah
Study design
Allocation
NA
Intervention model
SINGLE_GROUP
Primary purpose
BASIC_SCIENCE
Masking
NONE
Intervention model description
Participants will be tested before a 2-week limb immobilization period
Eligibility
Sex/Gender
ALL
Age
18 Years to 85 Years
Healthy volunteers
Yes
Inclusion criteria
1. Age between 18-35 and 60-85 yrs 2. Ability to sign informed consent 3. Free-living, prior to admission
Exclusion criteria
* History of cardiovascular disease (e.g., CHF, CAD, right-to-left shunt) * History of endocrine or metabolic disease such as hypo/hyperthyroidism and diabetes * History of kidney disease or failure * Vascular disease * Risk of DVT including family history of thrombophilia, DVT, pulmonary emboli, myeloproliferative diseases including polycythemia (Hb\>18 g/dL) or thrombocytosis (platelets\>400x103/mL), and connective tissue diseases (positive lupus anticoagulant), hyperhomocystinemia, deficiencies of factor V Leiden, proteins S and C, and antithrombine III * Use of anticoagulant therapy (e.g., Coumadin, heparin) * Elevated systolic pressure \>150 or a diastolic blood pressure \> 100 * Implanted electronic devices (e.g., pacemakers, electronic infusion pumps, stimulators) * Cancer or history of successfully treated cancer (less than 1 year) other than basal cell carcinoma * Currently on a weight-loss diet * Chronic systemic corticosteroid use (≥ 2 weeks) within 4 weeks of enrollment and for study duration (intra-articular/topical/inhaled therapeutic or physiologic doses of corticosteroids are permitted) * Androgens or growth hormone within 6 months of enrollment and for study duration (topical physiologic androgen replacement is permitted) * History of stroke with motor disability * A recent history (\<12 months) of GI bleed * History of liver disease * History of respiratory disease (acute upper respiratory infection, history of chronic lung disease) * Pregnancy as determined by a pregnancy test * Any staff members who report directly to an investigator or who report to someone who reports directly to an investigator. * Any other condition or event considered exclusionary by the PI and faculty physician
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Thigh Muscle Volume | baseline and after 2 weeks of leg immobilization | change in thigh muscle volume as determined by MRI |
| Leg Muscle Strength | baseline and after 2 weeks of leg immobilization | change in isometric leg extension strength as determined by a isokinetic dynamometer |
Countries
United States
Contacts
Primary ContactMicah Drummond, PhD
Outcome results
None listed