Dental Plaque
Conditions
Keywords
Saliva, Mouthwash, Green Tea, Oral bacteria
Brief summary
Given that the participation of oral microorganisms in the development of the most prevalent pathologies of the oral cavity is unquestionable, efforts have been made to create agents that allow controlling these bacteria. The objective of this work was to analyze the antibacterial effect of green tea infusion used as a mouthwash on saliva and bacterial plaque. The green tea infusion was prepared at 13% at approximately 90 ° C, being the same applied to 14 participants from the 1st to the 5th grade (experimental group); and to other 14 participants the placebo was applied (control group). The antibacterial effect was determined by counting the colony forming units (CFU) in the cultures of samples of bacterial plaque and saliva, taken before the application of the infusion and immediately after.
Detailed description
Oral diseases, including dental caries, is one of the most prevalent in the population. It is multifactorial and originates from the formation of the bacterial plaque and its non-elimination by physical and chemical hygiene methods. The use of chemical agents to aid in the control of Bacterial Plaque has been the subject of study in many investigations. Today there is a variety of elements that can be use to carry out proper oral hygiene, such as toothbrushes, toothpastes, dental floss and mouthwash, all of them in a wide variety. Often the lack of accessibility to these means problems in the hygiene in the population, either due to the lack of knowledge or the cost of obtaining them. Therefore, the objective of analyzing the antibacterial effect of the infusion of green tea (camellia sinensis) used as a mouthwash on saliva and bacterial plaque was presented. The proposed research topic is relevant, since if green tea (camellia sinensis) is found to produce positive effects on bacterial plaque, it would serve to prevent its formation, thus helping to improve dental hygiene with an easy acquisition product and lower cost compared to other mouthwashes offered in the market, constituting a natural mouthwash.
Interventions
OTHERGreen Tea mouthwash
an infusion at 13%, with 13 g of green tea (commercially divided) in 100 ml of saline solution, at a temperature of approximately 90 ° C, in sterile glass containers. Each application was 10 ml. applied for 1 minute.
OTHERPlacebo Mouthwash
distilled water
Sponsors
Julieta María Méndez Romero
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
PREVENTION
Masking
SINGLE (Subject)
Masking description
It was single blind, since the patient did not wat treatment was receiving. It was used a placebo.
Intervention model description
Experimental group: an infusion at 13%, with 13 g of green tea (commercially divided) in 100 ml of saline solution, at a temperature of approximately 90 ° C. Control group: distilled water, in sterile glass containers. Each application was 10 ml. applied for 1 minute.
Eligibility
Sex/Gender
ALL
Age
18 Years to No maximum
Healthy volunteers
Yes
Inclusion criteria
* Participants that signed the informed consent
Exclusion criteria
* allergic to green tea (Camellia sinensis) * ongoing medical or dental treatment that could interfere with the results.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Bacterial plaque Stage 2 (After intervention) | 1 minute after mouthwash use | The measure was done with the count of Colony forming units |
| Bacterial plaque Stage 1 (Before intervention) | thorough study completion, an average of 24 hours | The measure was done with the count of Colony forming units |
| Antimicrobial effect | up to 24 hours, thorough study completion | The measure was done with the difference in count of Colony forming units before and after intervention |
Countries
Paraguay
Outcome results
None listed