Periodontal Diseases
Conditions
Brief summary
Trefoil factor family (TFF) consists of a group of small peptides that have key roles in host immune response and repair of tissue damage. Interleukin (IL)-1β is a regulatory proinflammatory cytokine in periodontal inflammation. This study aimed to investigate the levels of TFF-1, TFF-3 and IL-1β in gingival crevicular fluid (GCF), saliva and serum of patients with gingivitis, stage 3 periodontitis and healthy individuals.
Detailed description
A total of one hundred participants were enrolled for the study. All individuals were assessed clinically and radiographically. Full-mouth clinical periodontal examinations were performed by the measurements of probing depth (PD), clinical attachment level (CAL), gingival index (GI), plaque index (PI) and bleeding on probing (BOP). All these measurements were recorded at six sites around each tooth with a manual periodontal probe. The participants were categorized into three groups: healthy controls (n=25), patients with gingivitis (n=25) and patients with periodontitis (n=50). Gingival crevicular fluid, saliva and serum samples were collected from each participant one day after the clinical periodontal measurements. TFF-1, TFF-3 and IL-1β levels of these biofluids were determined using the enzyme-linked immunosorbent assay.
Interventions
BIOLOGICALbiofluids
Gingival crevicular fluid, saliva and serum samples were obtained.
Sponsors
Aydin Adnan Menderes University
Study design
Observational model
CASE_CONTROL
Time perspective
CROSS_SECTIONAL
Eligibility
Sex/Gender
ALL
Age
25 Years to 49 Years
Inclusion criteria
* Systemically healthy individuals * No history of smoking * Individuals between the ages of 25-50 years * Having at least 20 natural teeth
Exclusion criteria
* Any systemic disorders (i.e. diabetes mellitus, cardiovascular diseases, rheumatoid arthritis, immunological disorders, collagen-metabolic diseases, cancer) * A history of smoking * Pregnancy or lactation * A history of surgical/non-surgical periodontal therapy in the past 1 year * Drug therapy (e.g. anti-inflammatory, antibiotic treatment or any other pharmacological treatment) in the previous 6 months
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Gingival crevicular fluid, saliva and serum TFF-1 and TFF-3 levels | one day after the clinical periodontal measurements | Gingival crevicular fluid, saliva and serum TFF-1 and TFF-3 levels of gingivitis and periodontitis patients as well as healthy individuals were analyzed to identify the potential role of these peptides in periodontal diseases. |
Countries
Turkey (Türkiye)
Outcome results
None listed