Bacterial Vaginoses
Conditions
Brief summary
The investigators aim to examine the endometrium with state of the art sequencing techniques to investigate the endometrial microbiota. The endometrial microbiota has been perceived to be sterile, however, this seems incorrect from recent studies. Thus, the primary outcome is to compare the rate of ascending infection from the vagina to the endometrium and to investigate which bacteria are capable of inhabiting these environments in healthy oocyte donors. These results will be compared to equivalent samples of IVF patients.
Interventions
None listed
Sponsors
Instituto Bernabeu
Peter Humaidan
Study design
Observational model
COHORT
Time perspective
CROSS_SECTIONAL
Eligibility
Sex/Gender
FEMALE
Age
18 Years to 35 Years
Healthy volunteers
No
Inclusion criteria
Oocyte donors meeting local criteria for donation. Written informed consent. Regular cycle
Exclusion criteria
Antibiotics within 1 month Vaginal products other than for menstrual hygiene within 1 month Uterine malformations(Septum, polyps, adenomyosis, fibroids) HIV, Hepatitis B or C positivity. HPV CIN 2 or higher. Chlamydia trachomatis positivity. Any uncontrolled concomitant disease (e.g. uncontrolled diabetes, uncontrolled hypertension etc.)
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Rate of ascending infection/sharing of bacteria in the genital tract | This outcome data is measured at the 1 day of oocyte retrieval. | vaginal, cervical and endometrial samples will be compared at the time of oocyte retrieval. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Next generation sequencing techniques and qPCR will be used to assess the microbiota.I.e. measure of relative abundances and absolute abundances | This outcome data is measured at the 1 day of oocyte retrieval. | qPCR to assess the microbiota. I.e. a quantitative measure Copies/mL. 16S V4 to investigate relative abundances |
Countries
Spain
Outcome results
None listed