Determination of the Levels of Lipocalin-2 and Semaphorin3A

Determination of the Levels of Lipocalin-2 and Semaphorin3A in Gingival Crevicular Fluid in Subjects With Different Periodontal Diseases

Status

Completed

Phases

Study type

Interventional

Source

ClinicalTrials.gov

Registry ID

NCT04390997

Enrollment

Registered

2020-05-18

Start date

2019-11-15

Completion date

2021-01-15

Last updated

2021-02-10

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Gingivitis, Periodontal Diseases, Periodontitis

Keywords

Periodontal Disease, Non-surgical Periodontal Treatment, GCF

Brief summary

The aim of this clinical trial is to investigate the concentrations of Lipocalin-2 and Semaphorin 3A levels in gingival crevicular fluid in subjects with different periodontal diseases. At the same time for the periodontitis group; the purpose of this study is to analyze the correlation of these proteins with clinical parameters at the first and third months after the nonsurgical periodontal treatment.

Detailed description

In our study, gingival crevicular fluid samples will be taken along with the measurement of all oral clinical parameters from sixty systemically healthy volunteers (20 periodontally healthy, 20 gingivitis, and 20 periodontitis). Gingival crevicular fluid samples will be taken again after the nonsurgical periodontal treatment for the periodontitis group in the first and third months. Lipocalin-2 and Semaphorin 3A levels will be determined by an enzyme-linked immunosorbent assay test (ELISA). Subsequent statistical analyses will be used to interpret protein levels among different groups and peridontitis group after the nonsurgical periodontal treatment. Possible significant differences may illuminate the future work with Lipocalin-2 and Semaphorin 3A. Also, these glycoproteins may help to develop different diagnostic methods or treatment strategies for future periodontal treatments.

Interventions

PROCEDURENon-surgical Periodontal Treatment

Non-surgical periodontal treatment includes scaling and root planning by using curettes and ultrasonic devices only in the periodontitis group.

Study design

Allocation

NON_RANDOMIZED

Intervention model

PARALLEL

Primary purpose

DIAGNOSTIC

Masking

SINGLE (Outcomes Assessor)

Eligibility

Sex/Gender

ALL

Age

18 Years to 70 Years

Healthy volunteers

Yes

Inclusion criteria

* Being systemically healthy * Having at least 20 teeth in the dentition, excluding third molars * Not using alcohol nor smoking * Not using medications * Not taking any antibiotics, anti-inflammatory, nor systemic corticosteroid drugs in the last six months * Not being in the lactation period nor pregnancy

Exclusion criteria

* Having any oral or systemic diseases * Using alcohol or smoking * Using medications * Taking any antibiotics, anti-inflammatory, or systemic corticosteroid drugs in the last six months * Being in the lactation period or pregnancy * Being treated with periodontal treatment in the last six months

Design outcomes

Primary

Measure	Time frame	Description
The level of Lipocalin-2	18 months	Investigating the concentrations of Lipocalin-2 level in gingival crevicular fluid in subjects with different periodontal diseases.
The level of Semaphorin 3A	18 months	Investigating the concentrations of Semaphorin 3A leves in gingival crevicular fluid in subjects with different periodontal diseases.

Secondary

Measure	Time frame	Description
Effects of non-surgical periodontal treatment on the levels of Lipocalin-2 and Semaphorin3A	18 months	Analyzing the correlation of Lipocalin-2 and Semaphorin3A with clinical parameters at the first and third months after the nonsurgical periodontal treatment

Countries

Turkey (Türkiye)

Outcome results

None listed

Source: ClinicalTrials.gov · Data processed: Feb 4, 2026