Pulp Necroses
Conditions
Keywords
Regenerative endodontics, Open apex, Immature necrotic teeth
Brief summary
The aim of the present study is to assess clinically and radio-graphically the regenerative potential of immature permanent teeth with necrotic pulp using blood clot and Platelet rich fibrin scaffolds.
Detailed description
30 immature necrotic permanent teeth in patients will be allocated and divided into two groups. Group 1 will be treated by regenerative procedure using blood clot as a scaffold and Group 2 will be treated with regenerative procedures using combined blood clot and Platelet rich fibrin as a scaffold.
Interventions
BIOLOGICALBlood clot scaffold
Bleeding will be induced in the canal by passing a size 30 sterile K-file 3 mm beyond the apex with the goal of having the entire canal filled with blood to the level of the cemento-enamel junction. Bleeding will be stopped just below the cemento enamel junction. Biodentine will be placed approximately 3-4 mm below the cementoenamel junction.
BIOLOGICALBlood clot scaffold combined with PRF
Platelet-rich fibrin will be prepared by drawing the patient blood into a 10mL test tube without the addition of an anticoagulant. To prevent the blood from coagulating after coming in contact with the glass tube, it will be centrifuged immediately using a table top centrifuge\*\* at 400 g force for 12 minutes. Then platelet-rich fibrin membrane will be placed into the canal space to a level 3 mm below the cemento-enamel junction using hand plugger following the induction of apical bleeding by passing a number 30 sterile hand file 3 mm beyond the apex of the tooth. Biodentine cap will be placed over the platelet-rich fibrin scaffold.
Sponsors
Alexandria University
Nourhan M.Aly
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
TREATMENT
Masking
SINGLE (Outcomes Assessor)
Masking description
The radiographic specialist and the statistician will be blinded to treatment group
Eligibility
Sex/Gender
ALL
Age
8 Years to 18 Years
Healthy volunteers
No
Inclusion criteria
* Necrotic incisors with immature roots * Pulp space not needed for post and core. * The patient has to be free from any coagulation disorders, with normal platelets count. * Good oral hygiene * Cooperative patient
Exclusion criteria
* Internal or external root resorption. * Root fracture and/or alveolar fracture. * Previous root canal treatment. * Medically compromised patients * Uncooperative patient
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Subjective pain assessment | 1 month | The patient will be asked about the presence of any pain or discomfort. Pain severity will be categorized as: no pain - mild pain - moderate pain - severe pain |
| Assessment of pulp vitality | 1 month | Pulp vitality will be assessed using thermal and electric pulp sensibility tests |
| Assessment of root development | 1 month | Continued root development will be assessed using cone beam computed tomography (CBCT) and compared with the contra-lateral healthy tooth |
| Assessment of the size of periapical radiolucency | 1 month | Size of the lesion will be assessed using the periapical index score. The PAI provides an ordinal scale of 5 scores ranging from 1 (healthy) to 5 (severe periodontitis with exacerbating features). |
Countries
Egypt
Outcome results
None listed