Comparative Study of Muc5ac and Muc2 Mucins in Tear Film Concentration in Surgical Treated Pterygia Patients

Comparative Study of Concentration of Muc5ac and Muc2 Mucins in Tear Film in Surgical Treated Pterygia With Amniotic Membrane Transplantation vs. Conjunctival Autograft

Status

Completed

Phases

Study type

Interventional

Source

ClinicalTrials.gov

Registry ID

NCT04385446

Enrollment

Registered

2020-05-13

Start date

2016-02-02

Completion date

2018-09-05

Last updated

2020-05-13

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Pterygium of Both Eyes

Keywords

pterygium, amniotic membrane, conjunctival autograft, ocular mucin

Brief summary

To identify and compare the differences in the concentration of the Muc2 and Muc5AC mucins in tear film in patients who underwent pterygium surgery using amniotic membrane transplantation and conjunctival autograft surgery.

Detailed description

Patients with nasal, primary pterygium bigger than 1 mm of corneal invasion with residence in Mexico City and older than 18 years were included. Under informed consent, patients were divided into two groups in a random fashion. Pterygium surgery was carried out with conventional technique performing amniotic membrane transplantation for group A, and conjunctival autograft for group B. Muc2 and muc5ac mucin concentration was measure min tear film by ELISA; samples were taken before surgery, at first, third and sixth month postoperatively. BUT was also measured on the same dates. Descriptive statistics and the U-Mann Whitney test for independent groups, considering a p≤0.05 as significant were used for analysis.

Interventions

PROCEDUREAmniotic membrane transplantation

Amniotic membrane transplantation was used as covering material during pterygium surgery.

Study design

Allocation

RANDOMIZED

Intervention model

PARALLEL

Primary purpose

SCREENING

Masking

DOUBLE (Subject, Outcomes Assessor)

Masking description

The patients did not know what kind of material was used in their surgery. The assessor who performed tear film analysis did not know what material was used in the patient.

Intervention model description

Two groups of patients in whom Conventional Pterygium surgery was performed Each patient was randomly assigned for either received amniotic membrane transplantation or conjunctival autograft as a recovering method in the pterygium removed area after surgery. Tear film Mucins were measured in both groups

Eligibility

Sex/Gender

ALL

Age

18 Years to No maximum

Healthy volunteers

Yes

Inclusion criteria

* Patients with nasal, primary pterygium bigger than 1 mm.

Exclusion criteria

* Diabetes mellitus, single eye.

Design outcomes

Primary

Measure	Time frame	Description
Ocular Tear film Mucin MUC5ac concentration.	The patients will be assed during six months	Using a capillary tube, tear film was collected en every patient. Then Concentration of mucin MUC5ac were measured by Elisa technique using a quantitative scale.
Ocular tear film Mucin MUC2 concentration.	The patients will be assed during six months	Using a capillary tube, tear film was collected en every patient. Then Concentration of mucin MUC2 were measured by Elisa technique using a quantitative scale.
Tear film breakup time.	The patients will be assed during six months	Tear film breakup time is the time between a complete blink and the appearance of the first random corneal dry spot and indicates relative tear film stability.

Secondary

Measure	Time frame	Description
Pterygium recurrence.	The patients will be assed during six months	Pterygium recurrence was defined as any fibrovascular proliferation from the original pterygium site from it was surgically removed.

Outcome results

None listed

Source: ClinicalTrials.gov · Data processed: Feb 4, 2026