Breast Milk Collection
Conditions
Brief summary
This study aims for characterization of different maternal, infant and environmental factors (e.g. maternal age, pregnancy duration, lactation period, baby gender, circadian rhythm, Holder pasteurisation, etc.) that influence human milk composition.
Detailed description
The study consists of three parts. In the first part the investigators aim to evaluate human milk macronutrient composition depending on the time after delivery and pregnancy duration. In the second part the investigators aim to evaluate the circadian variation of human milk macronutrient and energy content depending on pregnancy duration. In the third part the investigators aim to evaluate the influence of Holder pasteurisation on human milk macronutrient, metabolome and bioactive protein (lysozyme and lactoferrin) content.
Interventions
DEVICEAnalysis of human milk composition by mid-infrared spectrophotometry
Analysis of human milk macronutrient content was performed by mid-infrared spectrophotometry using a Miris Human Milk Analyser. An immune-enzymatic ELISA assays were used to estimate lysozyme and lactoferrin concentrations in human milk.
Sponsors
University of Cagliari
Vilnius University
Study design
Observational model
COHORT
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
FEMALE
Healthy volunteers
Yes
Inclusion criteria
having obtained informed consent; after a single-birth pregnancy; women who were not on a special diet; lactating mothers who could not breastfeed their newborns due to the baby's medical condition (either prematurity or disease) but who expressed milk. \-
Exclusion criteria
breastfeeding mothers; history of maternal diabetes, hepatitis B or C, HIV, tuberculosis, mastitis, or oncological disease; drug addicted. \-
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Quantification of total protein concentration in human milk | from the 2nd week, up to 2 months after delivery | Mid-infrared spectrophotometry |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Quantification of total lipids and carbohydrate in human milk | from the 2nd week, up to 2 months after delivery | Mid-infrared spectrophotometry |
| Quantification of lactoferrin in human milk | 14-16 days after delivery | An immune-enzymatic ELISA assay |
| Quantification of lysozyme in human milk | 14-16 days after delivery | An immune-enzymatic ELISA assay |
| Human milk metabolome analysis | 15-17 days after delivery | Nuclear Magnetic Resonance (NMR) analysis |
Countries
Lithuania
Outcome results
None listed