Covid-19, Diagnosis, Polymerase Chain Reaction, SARS-CoV-2
Conditions
Brief summary
The performance of 3 different sampling methods (2 nasopharyngeal swabs, 1 oropharyngeal swab) for the detection of SARS-CoV-2 with real-time reverse transcriptase polymerase chain reaction will be compared.
Detailed description
Thirty volunteers will be recruited from health care workers suspicious for Covid-19, who consult the ocupational physician for Covid-19 testing. For each volunteer, 3 swabs will be taken: * Flocked eSwab (Copan, Italy) of the oropharynx * Flocked nasopharyngeal eSwab (Copan, Italy) of the nasopharynx * Dry flocked nasopharyngeal Swab, placed in lysis buffer Each swab will be tested for the presence of SARS-CoV-2 with an in-house reverse-transcriptase real-time PCR (Egene). Performance of each method will be calculated.
Interventions
OTHERsamling of oropharynx and nasopharynx
Three different swabs will be taken of each participant: 1 oropharyngeal swab and 2 nasopharyngeal swabs
Sponsors
Jessa Hospital
Study design
Allocation
NA
Intervention model
SINGLE_GROUP
Primary purpose
DIAGNOSTIC
Masking
NONE
Masking description
Each participant get study number. Samples will be identified by the study number.
Intervention model description
Beside the regular sampling method of 1 nasopharyngeal swab, 2 extra swabs will be taken in each volunteer. Performance of the 3 methods for laboratory diagnosis by PCR of Covid-19 will be compared.
Eligibility
Sex/Gender
ALL
Healthy volunteers
Yes
Inclusion criteria
* health care workers with symptoms suspicious for Covid-19
Exclusion criteria
* none
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| sensitivity | 1 month | sensitivity of each sampling method for the detection of SARS-CoV-2 by RT-PCR |
Countries
Belgium
Outcome results
None listed