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Effects of a Multi-Ingredient Pre-Workout Supplement on Energy Expenditure During Running

Effects of One Versus Two Doses of a Multi-Ingredient Pre-Workout Supplement on Metabolic Factors and Perceived Exertion During Moderate-Intensity Running in Females

Status
Completed
Phases
NA
Study type
Interventional
Source
ClinicalTrials.gov
Registry ID
NCT04352998
Enrollment
12
Registered
2020-04-20
Start date
2016-05-16
Completion date
2016-05-16
Last updated
2020-04-20

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Healthy

Keywords

caffeine, substrate utilization, thermogenic, fat oxidation

Brief summary

The primary purpose of this study was to examine the acute effects of one versus two doses of a multi-ingredient pre-workout supplement on energy expenditure during moderate-intensity treadmill running. In addition, our second aim was to investigate the responses of associated metabolic factors (i.e. substrate utilization, measures of gas exchange), perceived exertion, and resting cardiovascular variables with one and two doses of the pre-workout supplement.

Detailed description

This study utilized a randomized, double-blind, placebo-controlled, within-subjects crossover design (Figure 1). Each subject was required to visit the laboratory on five occasions with 72-96 hours between sessions. During the first laboratory visit, each subject performed an incremental test on a treadmill to familiarize the subjects with the testing procedures. For the second laboratory visit, each subject performed an incremental treadmill test to exhaustion to determine their ventilatory threshold (VT). The third laboratory visit was completed in the morning (06:00-09:00) and required subjects to consume a standardized meal after fasting overnight (8 hours) and sat quietly for 30 minutes before baseline heart rate and blood pressure values were recorded. The subjects were then randomly assigned to ingest the supplement (one or two doses) or placebo and sit quietly for another 30 minutes. The ingredients of the supplement (Cellucor, Bryan, TX) are provided in Table 1. The placebo was non-energetic and controlled for similar appearance and taste. At the 15-minute and 30-minute post-ingestion periods (of the pre-workout supplement or placebo), resting heart rate and blood pressure were recorded for a second and third time, respectively. Subjects then performed a 30-minute constant-velocity treadmill run at 90% of their VT. The subjects then returned to the laboratory for their fourth and fifth visits to ingest the remaining substances (1-dose, 2-dose, or placebo) and undergo the same testing procedures (including time of day) as the third visit. Each subject recorded 2-day food logs (MyFitnessPal, Inc., USA) prior to each laboratory visit.

Interventions

DIETARY_SUPPLEMENTOne dose pre-workout condition

one dose of a multi-ingredient pre-workout supplement

DIETARY_SUPPLEMENTTwo dose pre-workout condition

two doses of a multi-ingredient pre-workout supplement

DIETARY_SUPPLEMENTPlacebo condition

one dose of a placebo

Sponsors

University of Wisconsin, La Crosse
CollaboratorOTHER
Mayo Clinic
CollaboratorOTHER
Nova Southeastern University
CollaboratorOTHER
Northern Illinois University
Lead SponsorOTHER

Study design

Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
OTHER
Masking
DOUBLE (Subject, Investigator)

Eligibility

Sex/Gender
FEMALE
Age
19 Years to 29 Years
Healthy volunteers
Yes

Inclusion criteria

* ran ≥ 16 km per week

Exclusion criteria

* (i) history of medical or surgical events, including cardiovascular disease, metabolic, renal, hepatic, or musculoskeletal disorders; (ii) use of any medication; (iii) use of nutritional supplements; (iv) habitual use of caffeine (≥ one cup of coffee or caffeinated beverage per day); or (v) participation in another clinical trial or investigation of another investigational product within 30 days prior to screening/enrollment.

Design outcomes

Primary

MeasureTime frameDescription
Energy expenditure30 minutes of exerciseenergy expenditure measured as kcals per minute

Secondary

MeasureTime frameDescription
substrate utilization30 minutes of exercisefat and carbohydrate oxidation
measures of gas exchange30 minutes of exerciseoxygen consumption, respiratory exchange ratio
perceived exertion30 minutes of exerciseratings of perceived exertion (RPE)
resting cardiovascular variablesbaseline and 30 minutes post-ingestionheart rate, systolic and diastolic blood pressure

Outcome results

None listed

Source: ClinicalTrials.gov · Data processed: Feb 4, 2026