Mycobacterium Pulmonary Infection
Conditions
Brief summary
Comparison of rapid detection methods of mycobacterial pathogens, GenSeizer v.s. PCR reverse hybridization.
Detailed description
The investigators developed a new platform called GenSeizer, which combines bioinformatics analysis of a large dataset with multiplex PCR-based targeted gene sequencing, to identify eleven major Mycobacterium species implicated in human disease. Simultaneous detection of certain antibiotic resistance genotypes, i.e., erm(41) and rrl in M. abscessus, is also feasible. This platform detected both reference strains and clinical isolates with a high degree of specificity and sensitivity. In this study, the investigators plan to verify the detection efficiency of GenSeizer in clinical samples (sputum, alveolar lavage fluid) and compare it with existing methods on the market (PCR reverse hybridization) and gold standard method (target sequencing after incubation)
Interventions
DIAGNOSTIC_TESTDetection method
Different methods for pathogen diagnosis
Sponsors
Shanghai Pulmonary Hospital, Shanghai, China
Study design
Observational model
COHORT
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
16 Years to 70 Years
Healthy volunteers
No
Inclusion criteria
* Suspected cases of mycobacterial lung infection
Exclusion criteria
* No sputum * Refused or unable to tolerate bronchoscopy
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Detection accuracy | through study completion, an average of 3 years |
Countries
China
Outcome results
None listed