Physical Activity
Conditions
Brief summary
The goal of the study is to examine the ability of resistance or aerobic exercise training to "imprint" skeletal muscle cells in a manner which confers long-term changes in this tissue which in-turn contribute to improved metabolic health and functional capacity through epigenetic regulation of novel exercise response genes. This study will also provide primary human skeletal muscle cells to the Molecular Transducers of Physical Activity Consortium (MoTrPAC) (NCT03960827) repository for future use.
Interventions
OTHERAdditional Muscle Biopsy Collection
Subjects will be asked if an additional biopsy from an existing incision can be obtained (i.e. additional needle insertion). For each biopsy required in the main MoTrPAC study (NCT03960827), a small needle will be used to inject some numbing medication (similar to what a dentist uses) in your thigh. A small incision (about 1/4 inch) will be made and a special needle will be used to collect 1 or 2 muscle samples (about the size of a pea).
Sponsors
AdventHealth Translational Research Institute
Wake Forest University
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
OTHER
Masking
NONE
Eligibility
Sex/Gender
ALL
Age
18 Years to No maximum
Healthy volunteers
Yes
Inclusion criteria
ADULT PARTICIPANT INCLUSION CRITERIA - SEDENTARY PARTICIPANTS * Willingness to provide informed consent to participate in the MoTrPAC Study -Willingness to undergo an extra needle insertion for the extra muscle tissue collection during the MoTrPAC muscle biopsy * Must be able to read and speak English well enough to provide informed consent and understand instructions * Aged ≥18 y * Body Mass Index (BMI) \>19 to \<35 kg/m2 * Sedentary defined as self-reporting no more than 1 day per week, lasting no more than 60 minutes, of regular (structured) EE \[e.g., brisk walking, jogging, running, cycling, elliptical, or swimming activity that results in feelings of increased heart rate, rapid breathing, and/or sweating\] or RE (resulting in muscular fatigue) in the past year * Persons bicycling as a mode of transportation to and from work \>1 day/week etc. are not considered sedentary * Leisure walkers are included unless they meet the heart rate, breathing, and sweating criteria noted above ADULT PARTICIPANT INCLUSION CRITERIA - HIGHLY ACTIVE PARTICIPANTS * Willingness to provide informed consent to participate in the MoTrPAC Study -Willingness to undergo an extra needle insertion for the extra muscle tissue collection during the MoTrPAC muscle biopsy * Must be able to read and speak English well enough to provide informed consent and understand instructions * Aged ≥18 y * BMI \>19 to \<35 kg/m2 * Comparator Participants * Highly Active Endurance Exercise (HAEE): defined as \>240 minutes/week of ET for \>1 year; this can include running, walking (brisk, power), cycling, elliptical, etc. which (at a minimum) results in increased heart rate, rapid breathing and sweating * Must include cycling at least 2 days/week * Highly Active Resistance Exercise (HARE): defined as RT of ≥3 upper and ≥3 lower body muscle groups ≥2 times/week for \>1 year; using a prescription sufficient to increase strength and muscle mass * Elite or Competitive Athletes: can be included, if they meet HAEE or HARE inclusion criteria * Potential participants are informed that use of performance enhancing drugs in the last 6 months is exclusionary * In addition to meeting HAEE or HARE inclusion criteria, all HA participants must meet all other
Exclusion criteria
defined in this protocol * Individuals who meet inclusion criteria for both HAEE and HARE are exclude
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Mitochondrial function | Week 12 visit | Tested via a Analysis of Covariance (ANCOVA) with baseline as covariate followed by post-hoc multiple comparisons. |
| Insulin-stimulated glycogen synthesis in human skeletal muscle cell cultures (HSkMC) | Week 12 visit | Tested via a Analysis of Covariance (ANCOVA) with baseline as covariate followed by post-hoc multiple comparisons. |
Countries
United States
Contacts
PRINCIPAL_INVESTIGATORLauren Sparks, PhD
Study Principal Investigator
Outcome results
None listed