Exercise, Resistance Training
Conditions
Keywords
resistance training, recovery, muscle hypertrophy, muscle signalling
Brief summary
Resistance training (RT) is one of the most important stimuli for muscle hypertrophy and it plays an important role on weight loss and fatty acid oxidation increase. RT affects anabolic pathways but the differences among various training techniques has been till now were poorly investigated. The aim of this study was to compare the effect of two different intensity of training, high-intensity interval resistance training (HIIRT) and traditional resistance training (TRT), on muscle signalling pathway.
Detailed description
Nine young healthy subjects performed HIIRT and TRT protocol in two different moments and with different legs. HIIRT technique consisted of three sets of: 6 repetitions at 6RM (6 repetitions maximum) and then 20 seconds of rest and 2/3 repetitions (until exhaustion) repeated for 3 times with 2'30 rest between sets; while TRT consisted of 3 sets of 15 reps with 75 sec of rest between sets. Biopsies from the vastus lateralis were taken at baseline (pre), immediately (0h) at the end of training, 6 hours (6h) and 24 hours (24h) after training. Western blot and RT-PCR mRNA (Reverse transcriptase-polymerase chain reaction) analysis were performed to assess muscle signalling pathway activation
Interventions
Subjects trained one leg with HIIRT
Subjects trained the other leg with HIIRT
Sponsors
University of Roma La Sapienza
University of Padova
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
BASIC_SCIENCE
Masking
SINGLE (Outcomes Assessor)
Masking description
The samples analysed were masked by a number without any indication of the arm.
Intervention model description
Same subjects serve as both the test and control arms which allows for within-subject comparison. Using a within-person design, participants trained each lower limb on separate days under two different training stimulus (HIIRT) and traditional training (TRT)
Eligibility
Sex/Gender
ALL
Age
20 Years to 30 Years
Healthy volunteers
Yes
Inclusion criteria
* moderate active * experience with resistance training
Exclusion criteria
* history of recent myocardial infarction, * severe cardiac arrhythmia, * unstable angina, * poorly controlled hypertension, * poorly controlled diabetes mellitus, * frequent or complex ventricular ectopy which might interfere with one's ability to adhere to exercise protocols
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| pAMPKThr172 in skeletal muscle tissue measured by immunoblot | 24 hours | measurement of pAMPKThr172 in skeletal muscle tissue taken by biopsy |
| pERK 1/2Thr202/Tyr204 in skeletal muscle tissue measured by immunoblot | 24 hours | measurement of pERK 1/2Thr202/Tyr204 in skeletal muscle tissue taken by biopsy |
| pS6Ser235/236 in skeletal muscle tissue measured by immunoblot | 24 hours | measurement of pS6Ser235/236 in skeletal muscle tissue taken by biopsy |
| p4EBP1Thr37/46 in skeletal muscle tissue measured by immunoblot | 24 hours | measurement of p4EBP1Thr37/46 in skeletal muscle tissue taken by biopsy |
| ACC (acetyl-coenzyme A carboxylase) phosphorylation in skeletal muscle tissue measured by immunoblot | 24 hours | ACC phosphorylation in skeletal muscle tissue taken by biopsy |
| IGF-1 mRNA (messenger ribonucleic acid) in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR) | 24 hours | IGF-1 mRNA in skeletal muscle tissue taken by biopsy |
| IGF-1ea (insulin like growth factor) mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR) | 24 hoursn | IGF-1ea mRNA in skeletal muscle tissue taken by biopsy |
| MGF (mechanical growth factor) mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR) | 24 hours | MGF mRNA in skeletal muscle tissue taken by biopsy |
| STARS mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR) | 24 hoursn | STARS mRNA in skeletal muscle tissue taken by biopsy |
| Myogenin mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR) | 24 hours | Myogenin mRNA in skeletal muscle tissue taken by biopsy |
| IL-6 (interleukin 6) mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR) | 24 hours | IL-6 mRNA in skeletal muscle tissue taken by biopsy |
| PGC1a4 (Peroxisome proliferator-activated receptor gamma coactivator 1-alpha 4) mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR) | 24 hours | PGC1a4 mRNA in skeletal muscle tissue taken by biopsy |
| PGC1a (Peroxisome proliferator-activated receptor gamma coactivator 1-alpha) mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR) | 24 hours | PGC1a mRNA in skeletal muscle tissue taken by biopsy |
| Atrogin mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR) | 24 hours | Atrogin mRNA in skeletal muscle tissue taken by biopsy |
| Beclin mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR) | 24 hours | Beclin mRNA in skeletal muscle tissue taken by biopsy |
| Myostatin mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR) | 24 hours | Myostatin mRNA in skeletal muscle tissue taken by biopsy |
Countries
Italy
Outcome results
None listed