Nephrotic Syndrome
Conditions
Keywords
urinary proteins, Brillouin spectroscopy, surface enhanced Raman spectroscopy, proteomics, non-invasive assessment
Brief summary
The proteinuria is widely recognized as a marker of kidney disease severity, as well as the predictor of renal function decline, cardiovascular outcomes, and all-cause mortality. However, the severity of kidney disease progression and these outcomes differs among patients with various amount of proteinuria. The potential mechanism underlining this disparity may be relevant to the quality and quantity of filtered proteins, especially their mechano-chemical properties such as physical viscosity and stiffness, amino-acid sequence, and molecular weight (low, middle and high molecular weight proteins). The goal of the current project is to develop and validate combined Brillouin & Surface-Enhanced Raman Scattering (SERS) Spectroscopy technique for simultaneous non-contact assessment of visco-elastic and chemical properties of urine proteins as biomarkers of kidney disease. Systematic studies of these properties of proteins in urine samples to be taken from diseased and healthy subjects will be cross-validated by Liquid Chromatography-Mass Spectrometry (LCMS). The project ultimately aims for the development of an optical spectroscopic sensor for rapid, non-contact monitoring of urine samples from patients in clinical settings.
Interventions
DEVICEhybrid Brillouin-Raman spectroscopy
Combination of Brillouin and surface-enhanced Raman (SERS) spectroscopy for assessment of both visco-elastic (i.e. mechanical) and chemical characterization of urinary proteins at the same time from the same excitation laser source
Sponsors
National Scientific Medical Center, Kazakhstan
Astana Medical University
Nazarbayev University
Study design
Observational model
COHORT
Time perspective
CROSS_SECTIONAL
Eligibility
Sex/Gender
ALL
Age
18 Years to 65 Years
Healthy volunteers
Yes
Inclusion criteria
* Both male and female patients with any ethnic group, aged between 18-65 years old * Newly diagnosed patients with glomerulonephritis and different range of proteinuria
Exclusion criteria
* patients aged \<18 and \>65 years, * eGFR\<60 ml/min, * pregnant females, * patients with diabetes mellitus, cancer, infectious diseases, and other life-treating comorbidities/conditions
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Non-invasive assessment of mechano-chemical properties of urine proteins by hybrid Brillouin-Raman spectroscopy in a patient with nephrotic syndrome | four weeks | Urine sample measurements by Brillouin and surface enhanced Raman (SERS) spectroscopy |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Non-invasive assessment of mechano-chemical properties of urine proteins by hybrid Brillouin-Raman spectroscopy in a patient with nephrotic syndrome | four weeks | Correlation and cross-validation of urinary protein Brillouin and SERS spectroscopy measurements by Liquid Chromatography-Mass Spectrometry (with proteomic measurements). |
Countries
Kazakhstan
Outcome results
None listed