Epiretinal Membrane
Conditions
Brief summary
ILM and ERM are routinely excised during surgery, as part of the surgical technique, and cultivation of hyalocytes will be performed ex vivo after surgery.
Detailed description
Hyalocytes, typically located 20 to 50μm anterior the internal limiting membrane (ILM), have the potential to contribute to development of epiretinal membranes (ERM) in presence of vitreoschisis. For macular holes, hyalocytes located on the ILM are hypothesized to contribute to the closure of the macular hole after application of the inverted ILM flap technique. ILM is routinely excised during surgical repair of macular holes. During peeling of ERM it is excised in case of persisting wrinkling of the retinal surface or partial en bloc excision with the ERM. Furthermore, excision of ILM during ERM peeling is associated with significantly lower recurrence rates of ERM and better Long term visual acuity, according to the results reported by Chang et al. Removal of ERM and ILM is part of the surgical routine during surgical repair of macular holes and peeling of epiretinal membranes. Therefore, cultivation of excised ERM and ILM samples does not increase risks for patients in any way. During study it is planned to cultivate hyalocytes for investigation of their role in macular hole closure.
Interventions
PROCEDUREmembrane peeling
ERM and ILM are peeled routinely during surgery
Sponsors
Vienna Institute for Research in Ocular Surgery
Study design
Allocation
NA
Intervention model
SINGLE_GROUP
Primary purpose
BASIC_SCIENCE
Masking
NONE
Eligibility
Sex/Gender
ALL
Age
18 Years to No maximum
Healthy volunteers
No
Inclusion criteria
* Macular hole or epiretinal Membrane
Exclusion criteria
* Pregnancy (pregnancy test will be taken preoperatively in women of reproductive age)
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Proliferation of Hyalocytes | 9 days | hyalocytes from ERM and ILM will be cultured and counted |
Countries
Austria
Outcome results
None listed