Exercise Training
Conditions
Brief summary
The purpose of this study is to compare the impact of augmenting exercise intensity and augmenting exercise frequency on peak work rate. Participants will complete exercise tests and provide 8 skeletal muscle samples following a within-subjects randomized crossover design utilizing single-leg cycling. Both training periods will be 4 weeks long and skeletal muscle biopsies will be collected from both legs before and after each training period. All exercise sessions will be supervised, take place in the investigator's laboratory, and occur on stationary bikes.
Interventions
BEHAVIORALFrequency comparison
One leg assigned to low-frequency low-intensity (3 days per week of 11 x 1 min @ 73% peak work rate interspersed with 1-minute periods of active recovery). The other leg assigned to high-frequency low-intensity (5 days per week of 11 x 1 min @ 73% peak work rate interspersed with 1-minute periods of active recovery).
BEHAVIORALIntensity comparison
One leg assigned to low-frequency low-intensity (3 days per week of 11 x 1 min @ 73% peak work rate interspersed with 1-minute periods of active recovery). The other leg assigned to low-frequency high-intensity (3 days per week of 8 x 1 min @ 100% peak work rate interspersed with 1-minute periods of active recovery).
Sponsors
Queen's University
Brendon Gurd, PhD
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
BASIC_SCIENCE
Masking
SINGLE (Outcomes Assessor)
Eligibility
Sex/Gender
ALL
Age
18 Years to 30 Years
Healthy volunteers
Yes
Inclusion criteria
* Recreational active (\<3 hours per week of aerobic exercise) * Willing to provide skeletal muscle biopsies
Exclusion criteria
* Cardiovascular or metabolic disease * Athletes or individuals exceeding 3 hours per week of aerobic exercise * Smokers
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Peak work rate | Before exercise training on two days (~7 and 6 days before first training session; ~24 hours apart), and after exercise on two days (~48-96 hours after the last training session; ~24 hours apart) | Change in Highest work rate achieved (30s period) during incremental exercise tests |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Peak oxygen uptake | Before exercise training on two days (~7 and 6 days before first training session; ~24 hours apart), and after exercise on two days (~48-96 hours after the last training session; ~24 hours apart) | Change in Highest oxygen uptake achieved (30s period) during incremental exercise tests |
| Maximal citrate synthase activity | Before exercise training (~7 days before first training session), and after exercise training (~48-72 hours after the last training session) | Change in maximal citrate synthase activity |
Countries
Canada
Outcome results
None listed