Overweight and Obesity
Conditions
Brief summary
A study to address the effects of early-day fasting on fat tissue mobilization in comparison to a typical isocaloric Mediterranean and a low-carbohydrate breakfast.
Detailed description
The study design meets a cross-over pattern with 3 different morning visits of 6 hour duration each. After an informed consent is received from the participant, a crude medical history, physical examination and collection of somatometric data through bio-impendance analysis will take place. An intravenous catheter will be placed in the participants forearm. At zero time point, depending on study visit, participants will either (A) remain fasted (B) start consuming ta pre-prepared zero-carbohydrate 500 kcal test meal (C) start consuming ta pre-prepared 500 kcal test meal with a composition conforming to a standard Mediterannean breakfast. Participants will be given 10 minutes to consume the meal. Blood samples will be drawn and visual analogue scales (VAS) for hunger and satiety will be filled in at the following time points (min): -15, 0, 15, 30, 60, 90, 120, 180, 240, 300, 360.
Interventions
OTHERfasting
6-hour fasting
OTHERLow carbohydrate
Consumption of a zero-carbohydrate breakfast
OTHERMediterranean
Consumption of a Mediterranean-type breakfast
Sponsors
National and Kapodistrian University of Athens
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
OTHER
Masking
NONE
Eligibility
Sex/Gender
ALL
Age
18 Years to 45 Years
Healthy volunteers
Yes
Inclusion criteria
* BMI 18-25 kg/m2 * Willfulness to participate
Exclusion criteria
* Overweight or obesity (BMI\>25 kg/m2) * Type 1 or 2 diabetes mellitus * Uncontrolled thyroid disorders
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Mobilisation of adipose tissue | 6 hours | Area Under the Curve (AUC) of free fatty acid (FFA) and beta-Hydroxybutyrate (bOB) concentration |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Mobilisation of gut peptides | 6 hours | AUC of Ghrelin, Peptide YY and glucagon-like peptide -1 (GLP-1) concentration |
| Degree of appetite suppression | 6 hours | Visual Analogue Scale score (0-10, 0 equals minimal and 10 equals highest) for appetite |
| Degree of satiety induction | 6 hours | Visual Analogue Scale score (0-10, 0 equals minimal and 10 equals highest) for satiety |
Countries
Greece
Outcome results
None listed