Children, Dental Caries, Oxidative Stress, Passive Smoking
Conditions
Brief summary
The aim of the present study was to determine the effect of passive smoking on the levels of salivary TAS, TOS, LL-37 and dental status in children.In the study the parents of children were asked to fill out a complete survey about smoking habits. After filling out the questionnaire, according to the survey results, a total of 180 children were included to the study as follows; 90 children exposed to passive smoking, and 90 children in the control group (unexposed controls). Also demographic data were recorded (age, gender, parental education levels, child's tooth-brushing habit and child's daily dietary sugar exposure, family income). Dental examination of children were performed and caries prevalance of the patients were recorded. Unstimulated saliva samples were collected from children. Saliva 'cotinine levels' which are expected to increase in passive smoking group; 'antimicrobial peptide LL-37' and oxidative stress markers 'total antioxidant status' (TAS), and' total oxidant status' (TOS) were evaluated by using Enzyme-linked immunosorbent assay (ELISA). The obtained data parameters of the two groups were evaluated and comparison was performed.
Interventions
DIAGNOSTIC_TESTtaking saliva samples to determine TAS, TOS and LL-37
Sponsors
Kırıkkale University
Merve Erkmen Almaz
Study design
Observational model
CASE_CONTROL
Time perspective
CROSS_SECTIONAL
Eligibility
Sex/Gender
ALL
Age
6 Years to 12 Years
Healthy volunteers
Yes
Inclusion criteria
-Systemically healthy children
Exclusion criteria
* The presence of orthodontic / intraoral appliance * Any disease or condition known to affect defense system * Uncooperative children
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| salivary oxidative stress values: TAS level | 1 day (once after the dental examination) | Unstimulated saliva samples were collected from children. |
| salivary oxidative stress values: TOS level | 1 day (once after the dental examination) | Unstimulated saliva samples were collected from children. |
| salivary LL-37 level | 1 day (once after the dental examination) | Unstimulated saliva samples were collected from children. |
| salivary cotinine levels | 1 day (once after the dental examination) | Unstimulated saliva samples were collected from children for describing passive smoking children. |
Outcome results
None listed