Kidney Transplant; Complications, Liver Transplant; Complications, Microbial Colonization, Stem Cell Transplant Complications, Transplantation Infection
Conditions
Keywords
Microbiome, Gut microbiome, Stem cell transplantation, Kidney transplantation
Brief summary
This is a pilot study designed to investigate the alterations in the gut microbiome that occur during the course of kidney transplantation, liver transplantation, allogeneic hematopoietic stem cells and mesenchymal stem cells transplantation in association with the clinical outcomes.
Detailed description
This is a pilot study designed to investigate the dynamic changes in the intestinal microbiome that occur during the course of kidney transplantation, liver transplantation, allogeneic HSCs and mesenchymal stem cells transplantation. Four patient cohorts will be followed. Cohort A: patients receiving kidney transplantation. Cohort B: patients receiving hematopoietic stem cells transplantation. Cohort C: patients receiving mesenchymal stem cells transplantation. Cohort D: patients receiving liver transplantation. Included participants are defined as patients with at least three analyzable stool samples including a baseline sample before the transplantation.
Interventions
DIAGNOSTIC_TESTIntestinal microbiome new generation sequencing
Fecal samples collected at baseline pre-transplant and post-transplant, new generation 16s RNA sequencing performed.
Sponsors
Minsk Scientific-Practical Center for Surgery, Transplantation and Hematology
Study design
Observational model
COHORT
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Healthy volunteers
No
Inclusion criteria
* Participants enrolled into kidney transplantation or HSCs transplantation or MSCs transplantation or liver transplantation programs with fecal samples collected over the course of their initial transplant hospitalization. * Be willing and able to provide written informed consent for the study.
Exclusion criteria
* Patients were excluded from the study if their first sample was not collected prior to the start of transplantation procedure. * Samples were excluded from the analysis if they were not aliquoted and stored frozen within 24 hours of collection.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Gut microbiome composition | 1 month post-transplant | Dynamics of gut microbiome composition over the course of transplantation (Shannon diversity index, Simpson index, abundance of main taxonomic phyla) |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Infectious complications after transplantation | 2 years post-transplant | Incidence of infectious complications |
| Organ or tissue rejection | 2 years post-transplant | Incidence of organ or tissue rejection |
Countries
Belarus
Outcome results
None listed