Cancer of Colon, Metastasis to Liver
Conditions
Brief summary
Analyze the dynamics of incorporation of 5-fluorouracil into cancer cell ribosomes from liver metastases of patient with metastatic colon cancer.
Detailed description
This is a monocentric, prospective, non-interventional cohort study in adult patients with metastatic colon cancer with hepatics metastasis. Tumour samples collected for this study include: * A fragment of the previously operated primary tumor: FFIP block and/or archived frozen tumor if available. * A fragment of the liver metastasis(s) performed as part of standard metastasis surgery, this fragment will include a tumor area and a healthy area within the margin of excision. These samples collected in a fresh state will be analyzed immediately.
Interventions
PROCEDUREScheduled and planned surgery
Collection of tumour samples from samples collected as part of standard patient management. Diagnostic pathological analysis will be a priority. These samples will be taken by experienced staff during the hospital visit scheduled as part of standard care.
Sponsors
Centre Leon Berard
Study design
Observational model
CASE_ONLY
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
18 Years to No maximum
Healthy volunteers
No
Inclusion criteria
* Patient with metastatic colon cancer operated on and followed up at the Centre Leon Berard for liver metastasis. * Patient affiliated to social security
Exclusion criteria
* Other pathologies that may interfere with biological evaluation, in particular known hepatic cirrhosis, whatever its origin, other liver overload diseases (hemochromatosis, Wilson's disease), but excluding steatosis (which can be chemo-induced).
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| 5-FU in ribosome | Up to 24 months | Rate of incorporation of 5-fluorouracil into ribosomes of tumor tissues and adjacent healthy tissues before surgery and after surgery. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Methylation profile of ribosomal RNAs | Up to 24 months | Analyze on a large scale the alterations of the 2'-O-methylation profile of ribosomal RNAs before surgery and after surgery |
| Transcriptomic and proteomic profile | Up to 24 months | Define a transcriptomic and proteomic profile of the collected samples before surgery and after surgery. |
| Specific molecular signature | Up to 24 months | Combination of the number of 5-fluorouracil molecules incorporated per rRNA molecule, the methylation profile and the transcriptomic profile before surgery and after surgery. |
Countries
France
Outcome results
None listed