Atopic Dermatitis, Bacterial Toxins, Cytokines, Immune Response, Staphylococcus Aureus, T Cells Subsets
Conditions
Brief summary
Atopic dermatitis (AD) is the most common chronic inflammatory skin disease. Clinical studies have demonstrated a link between staphylococcal skin colonization and the pathogenesis of AD, but the implication of bacterial virulence factors remains largely uncharacterized. Finally, AD is often associated with herpes simplex skin infections. The aim of this project is to investigate the role of staphylococcal toxins in the exacerbation and maintenance of atopic skin inflammation and in the occurrence of infectious complications such as eczema herpeticum.
Interventions
OTHERSkin biopsies and blood samples
Two skin biopsies and 30 ml of blood will be collected.
Sponsors
Poitiers University Hospital
Study design
Allocation
NA
Intervention model
SINGLE_GROUP
Primary purpose
BASIC_SCIENCE
Masking
NONE
Eligibility
Sex/Gender
ALL
Age
18 Years to No maximum
Healthy volunteers
No
Inclusion criteria
* Patients with moderate AD (SCORAD between 25 and 50) or severe AD (SCORAD\> 50) * Skin lesions in the forearms * Free subject, without neither guardianship, wardship nor subordination * Patient with Social Security * Informed and signed consent by the patient after clear and loyal information on the study
Exclusion criteria
* Age \< 18 year-old * Patients with mild AD (SCORAD \< 25) * Patients without skin lesions in the forearms * Patients treated with dermocorticoid or calcineurin inhibitor for less than two weeks * Patients under systemic treatment : Methotrexate, Ciclosporin, Mycophenolate Mofetil, Azathioprine, general corticosteroids for less than 4 weeks * Patients under biological treatment : Dupilumab for less than 5 half-lives * Patient without Social Security * Pregnant and nursing women
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Quantification of S. aureus colonization level and characterization of bacterial virulence profile in AD lesions. | 2 hours (with consultation and sample) | Biological data obtained from lesional skin will be compared with those of healthy skin. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Determination of the inflammatory profile of skin and blood during AD. Definition of the seric cytokine signature characteristic of AD. Characterization of the phenotype and function of the lymphocytic infiltrate T during AD. | 2 hours (with consultation and sample) | Biological data obtained from lesional skin will be compared with those of healthy skin. |
Countries
France
Outcome results
None listed