Analysis of T Cell Metabolism in Acute Myeloid Leukemia Patients

Analysis of T Cell Metabolism and Immune Phenotype During the Course of Acute Myeloid Leukemia

Status

Completed

Phases

Unknown

Study type

Observational

Source

ClinicalTrials.gov

Registry ID

NCT04259372

Enrollment

Registered

2020-02-06

Start date

2016-01-01

Completion date

2019-12-31

Last updated

2020-02-06

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Acute Myeloid Leukemia

Brief summary

The objective of this study was to analyze the T cell metabolism and immune phenotype in AML patients during the course of the disease before and after allo-HCT.

Detailed description

Patients who experience relapse of acute myeloid leukemia (AML) after allogeneic hematopoietic cell transplantation (allo-HCT) have a dismal prognosis. Infusion of donor T cells can induce graft-versus-leukemia (GVL) effects, indicating the importance of intact T cell function for leukemia control. Recent studies have shown the importance of metabolic fitness for an effective T cell response. In this study we have analyzed the T cell metabolism and immune phenotype in AML patients at different time points before and after allo-HCT.

Interventions

None listed

Study design

Observational model

COHORT

Time perspective

PROSPECTIVE

Eligibility

Sex/Gender

ALL

Age

18 Years to No maximum

Healthy volunteers

Yes

Inclusion criteria

* confirmed diagnosis of AML * age ≥ 18 years * peripheral blood sample available * written informed consent * ability to understand the nature of the study and the study related procedures and to comply with them

Exclusion criteria

* age \< 18 years * lack of informed consent * patients that cannot be classified in one of the 3 groups

Design outcomes

Primary

Measure	Time frame	Description
T cell metabolism	4 years	Extracellular acidification rate (ECAR) and oxygen consumption rate (OCR) determined by live-cell metabolic assay using the Seahorse Analyzer
T cell phenotype	4 years	T cell cytokine and effector molecule production

Secondary

Measure	Time frame	Description
Metabolite abundance in serum	4 years	Serum metabolites as measured by mass spectrometry

Outcome results