Cervix Cancer, Oxidative Stress
Conditions
Keywords
thiol disulfide hemostasis, screening, early diagnosis
Brief summary
The aim of this research is to evaluate thiol-disulfide balance in cervix cancer patients
Detailed description
To date various oxidative stress parameters were studied in patients with cervix cancer, however dynamic thiol disulfide hemostasis has not been previously investigated. The thiol-disulfide balance in the circulation will be measured by the automated system created by Erel & Neselioglu (1). References 1- Erel O, Neselioglu S. A novel and automated assay for thiol/disulphide homeostasis. Clin Biochem 2014;47:326-32. doi:10.1016/j.clinbiochem.2014.09.026.
Interventions
DIAGNOSTIC_TESTDisulphide(μmol/L)
Aforementioned oxidative stress markers will be measured via Cobas c501(Roche Diagnostics, Indianapolis, IN, USA) used with spectrophotometry
DIAGNOSTIC_TESTTotal Oxidant Capacity(μmolH2O2Equiv/L)
Aforementioned oxidative stress markers will be measured via Cobas c501(Roche Diagnostics, Indianapolis, IN, USA) used with spectrophotometry
DIAGNOSTIC_TESTIschemia Modified Albumin(IU/mL)
The serum Ischemia Modified Albumin concentrations were measured as described by Bar-Or et al. (Bar-Or et al. 2000).
Sponsors
Muğla Sıtkı Koçman University
Study design
Observational model
CASE_CONTROL
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
FEMALE
Age
18 Years to No maximum
Healthy volunteers
Yes
Inclusion criteria
• Histopathologically confirmed diagnosis of cervix cancer
Exclusion criteria
* pulmonary disease * pulmonary hypertension * cardiac dysfunction * renal dysfunction * liver disease * chronic ischemia * systemic inflammation * concomitant malignancy * patients who use vitamin A,C or E (antioxidant vitamins) * patients who use smoke * patients who use drink alcohol * patients who use addictive for any drug
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| native thiol/total thiol ratio | preoperative in the morning or preprandial for healthy volunteers | native thiol/total thiol ratio X (100 ) |
| serum disulphide level | preoperative in the morning or preprandial for healthy volunteers | serum disulphide level in μmol/L |
| serum total thiol level | preoperative in the morning or preprandial for healthy volunteers | serum total thiol level in μmol/L |
| the disulphide/native thiol ratio | preoperative in the morning or preprandial for healthy volunteers | the disulphide/native thiol ratio X (100 ) |
| disulphide/total thiol ratio | preoperative in the morning or preprandial for healthy volunteers | disulphide/total thiol ratio X (100 ) |
| serum native thiol level | preoperative in the morning or preprandial for healthy volunteers | serum native thiol level in μmol/L |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| total antioxidant capacity (TAC) | preoperative in the morning or preprandial for healthy volunteers | TAC in mmol trolox equivalent/L |
| Oxidative stress index (OSI) | preoperative in the morning or preprandial for healthy volunteers | OSI in mmol trolox/L. |
| ischaemia-modified albumin | preoperative in the morning or preprandial for healthy volunteers | ischaemia-modified albumin in IU/mL |
| total oxidant capacity (TOC) | preoperative in the morning or preprandial for healthy volunteers | TOC in mmol H2O2 equivalent/L |
Countries
Turkey (Türkiye)
Outcome results
None listed