Oxidative Stress
Conditions
Brief summary
Uncontrolled and prolonged oxidative stress plays an important role in the onset and progression of cardiovascular disease, hyperlipidemia, diabetes, and various cancers. Given that many diseases can start as early as childhood, eating patterns in childhood and preventing oxidative damage can have beneficial long-term health effects. Antioxidant-rich foods can slow down the progression of chronic diseases. In Slovenian kindergartens (and schools) children consume up to 70% of their daily energy and nutritional needs, so what is offered to them is very important. This study will evaluate the hypothesis that providing an antioxidant-rich diet in kindergartens can result in the reduction of biomarkers of oxidative stress.
Interventions
DIETARY_SUPPLEMENTAntioxidant
Antioxidant-rich diet (added selected types of fruits, vegetables, nuts, cereals and oils)
Sponsors
University of Ljubljana
University of Ljubljana, Faculty of Medicine
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
SUPPORTIVE_CARE
Masking
NONE
Eligibility
Sex/Gender
ALL
Age
5 Years to 7 Years
Healthy volunteers
Yes
Inclusion criteria
* • Healthy children aged 5-6 who will attend the compulsory medical examination before entering primary school
Exclusion criteria
* • Children with chronic conditions (e.g. diabetes, asthma) * Children with allergies to food (e.g. gluten, egg, milk, lactose intolerance)
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Oxidative stress biomarkers | 2 weeks | Change in oxidative stress biomarkers of lipids (malondialdehyde (MDA), and four F2 - isoprostane isomers, namely 8 -iso-prostaglandin F2α (8-PGF2α), 11ß- prostaglandin F2α (11-PGF2α), 15 (R)-prostaglandin F2α (15-PGF 2α) and 8-iso, 15 prostaglandin F2α (8,15-PGF2α) ; proteins (o,o'-dityrosine (diY) and DNA 8-Hydroxy-2'-deoxyguanosine (8-OhdG) between day 1 and day 15 of the intervention diet, measured by HPLC-MS/MS |
Countries
Slovenia
Outcome results
None listed