Urinary Tract Infections
Conditions
Brief summary
* to describe the profile of uropathogenic E coli from Egyptian pregnant women with UTIs and a symptomatic bacteriuria. * to determine the susceptibility pattern of our local isolated UPEC strains which is essential for optimal management of UTI. * to evaluate the different virulence genes in UPEC isolates and their association with antibiotic resistance. * to track common UPEC serogroups. * to investigate the biofilm formation and the relationship between virulence genes and biofilm formation in UPEC strains isolated from patients.
Detailed description
Urinary tract infection represents the most common bacterial infection in pregnancy and the third common cause of human infection after respiratory and intestinal infections. The infection can be life threatening and associated with serious complications. UTI can be associated with defined symptoms'symptomatic' or without symptoms 'asymptomatic'. Asymptomatic bacteriuria is defined as the presence of \>100000 colony forming units/ml of urine of a single pathogen in two consecutive midstream clean catch urine specimen or on catheterization specimen from an individual without signs or symptoms associated with urinary or genital organs. Asymptomatic bacteriuria can lead to acute pyelonephritis in30%of pregnant women and complications such as early delivery, increase risk of hypertension, pre-eclampsia, low birth weight and postpartum endometritis. Ecoli accounts for most cases of symptomatic and asymptomatic bacteriuria in women, representing70 \_90 %of the cases. The important virulence factors of uropathogenic ecoli can be broadly divided into two groups :bacterial cell surface factors and secreted factors. The emergence of drug resistant microorganism among Uropathogenic Escherichia coli strains increases the serious threat to global health. Ecoli often acquired genes coding for antibiotic resistance, beta-lactamase enzymes are the most frequent and play a key role, conferring resistance of bacteria to beta lactam antibiotic group such as penicillin and cephalosporins. Therefore, knowledge regarding local prevalence of uropathogenic ecoli and antimicrobial resistance is essential for optimal management of UTI.
Interventions
OTHERTalking a urine sample
Just taking a urine sample
Sponsors
Assiut University
Study design
Observational model
CASE_ONLY
Time perspective
CROSS_SECTIONAL
Eligibility
Sex/Gender
FEMALE
Age
18 Years to 45 Years
Healthy volunteers
No
Inclusion criteria
* pregnant women who diagnosed through clinical examination and urine analysis. 3 groups : group 1 pregnant women with cystitis Group 2 pregnant women with Pyelonephritis Group 3 pregnant women with asymptomatic bacteriuria
Exclusion criteria
* pregnant women on antibiotics \_Patients with any anatomical abnormalities in the urinary tract
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| The percentage of virulence genes in different isolated UPEC strains | Three months | Measurment of virulence genes of Escherichia coli strains by molecular diagnosis as PCR technique to determine relationship between clinical presentation of urinary tract infection and distribution of virulence genes of strains |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Correlation between the virulence genes and antibiotic resistance | Seven months | Measurment of virulence genes of escherichia coli strains by molecular diagnosis as PCR technique,and determine antibiotic susceptibility of ecoli strains between different groups of women to determine relationship between distribution of virulence genea and antibiotic susceptibility of strains |
Contacts
Primary ContactNoura Samir Hussein, Demonstrator
Outcome results
None listed