Healthy
Conditions
Brief summary
The aim of this project is to study the effect of a bout of exercise on the metabolomic profile of plasma and feces as well as its influence on the intestinal microbiota.
Detailed description
The practice of physical exercise has numerous health benefits, preventing the appearance and development of cardiovascular diseases and various types of cancer. Exercise causes changes in the vascular, muscular and pulmonary systems, etc. One of the benefits that can result from exercise modification of the intestinal microbiota and its metabolic profile. In order to identify the potential changes that exercise has on the intestinal microbiota and its metabolic profile, the purpose of this study is to identify changes in the plasma and fecal metabolome and in fecal microbiota associated with a bout of exercise
Interventions
OTHERProlonged Exercise session
After a standardized warm-up of 10 minutes of continuous running on a treadmill at 60% of their maximum heart rate (HRmax), subjects will perform a maximum oxygen consumption test (VO2max) with a gas analyzer (UltimaTM Series, MGC Diagnostic Corporation, St. Paul, MN, USA. The protocol will start with a slope of 1% at a speed of 10 km/h, with increments of 0.3 km/h every 30 s until volitional exhaustion. Finally, ten minutes after the treadmill test, volunteers will run 1 kilometer (t1km) as fast as possible and the time needed to cover the distance will be recorded.
Sponsors
Universidad Europea de Madrid
Study design
Allocation
NA
Intervention model
SINGLE_GROUP
Primary purpose
BASIC_SCIENCE
Masking
NONE
Eligibility
Sex/Gender
MALE
Age
18 Years to 45 Years
Healthy volunteers
Yes
Inclusion criteria
* 18-45 years of age * high physical condition (VO2 ≥ 55 ml/kg/min) * BMI 18-25 kg/m2
Exclusion criteria
* antibiotics intake during 3 months prior to the study, * smoking * nutritional complements * ergogenic complements * prebiotics * probiotics * vegetarian or vegan diet * chronic medication * gastrointestinal surgery * Any diagnosed disease
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Plasma metabolome profile | Changes from baseline to 30 min after the exercise intervention | Abundance of blood metabolites, metabolic networks, and metabolic pathways activity |
| Fecal microbiota | Changes from baseline to 30 min after the exercise intervention | Abundance of gut microbial taxa, communities, and metabolic pathways activity |
Countries
Spain
Outcome results
None listed