Atherosclerosis, Inflammation, Sleep Apnea
Conditions
Keywords
PTP1B, macrophage
Brief summary
Obstructive sleep apnea (OSA) syndrome is associated with increased vascular dysfunction and atherosclerosis. Especially, it has been shown that OSA associated intermittent hypoxia represents a pro inflammatory stimulus resulting in macrophage polarization. Protein tyrosine phosphatase 1B (PTP1B) is a negative regulator of insulin signaling pathways involved in atherosclerosis. It has been shown that myeloid PTP1B deficiency protects against atherosclerosis. As hypoxia has also been shown to increase PTP1B expression and activity, this study will evaluate the myeloid PTP1B expression and activity in patients with OSA as compared to controls and will investigate myeloid PTP1B involvement in the vascular pro inflammatory precess described in OSA.
Interventions
OTHERmyeloid PTP1B expression analysis
peripheral blood macrophage PTP1B expression
Sponsors
University Hospital, Angers
Study design
Allocation
NON_RANDOMIZED
Intervention model
PARALLEL
Primary purpose
OTHER
Masking
NONE
Intervention model description
patients with OSA 'apnea hypopnea index ≥ 15 per hour) as compared to controls (an apnea hypopnea index \< 15 per hour)
Eligibility
Sex/Gender
ALL
Age
18 Years to No maximum
Healthy volunteers
No
Inclusion criteria
* Overnight polysomnographic recording for suspected OSA * Apnea hypopnea index ≥ 15 per hour for OSA patients * Apnea hypopnea index \< 15 per hour for non OSA patients
Exclusion criteria
* past history of cardiovascular diseases * diabetes * dyslipidemia * hypertension * obesity (body mass index \> 35 kg/m2 * past history or ongoing inflammatory diseases * cognition impairment
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| myeloid PTP1B expression | baseline | Peripheral blood will be collected from OSA and non OSA patients. Macrophage will be isolated by specific centrifugation. PTP1B expression will be assessed by Western Blot. |
| myeloid PTP1B activity | baseline | Peripheral blood will be collected from OSA and non OSA patients. Macrophage will be isolated by specific centrifugation. PTP1B activity will be assessed by specific colorimetric assay. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| PTP1B exosomal expression. | baseline | Peripheral blood will be collected from OSA and non OSA patients. Exosomes will be isolated by consecutive centrifugations. PTP1B expression will be assessed by Western Blot. |
| in vitro PTP1B implication on macrophage inflammatory response | baseline | Macrophage isolated from patients will be stimulated by pro inflammatory agents (IL6, TNF alpha) in the presence and absence of PTP1B inhibitor (MSI-1436). The inflammatory response will be assessed by measuring inflammatory cytokines in the supernatant by ELISA. |
Countries
France
Outcome results
None listed