Healthy, Obese
Conditions
Brief summary
Researchers are trying to better understand the relationship between inflammation in adipose tissue (AT) and insulin resistance.
Interventions
PROCEDUREFat Tissue Biopsy
If subjects are scheduled for a surgical procedure a fat sample will be collected during the beginning of the surgery. If subjects are not scheduled for a surgical procedure, a small sample of subcutaneous (superficial) fat tissue from subjects abdomen area will be collected.
Sponsors
Mayo Clinic
Study design
Allocation
NON_RANDOMIZED
Intervention model
PARALLEL
Primary purpose
BASIC_SCIENCE
Masking
NONE
Eligibility
Sex/Gender
ALL
Age
30 Years to 50 Years
Healthy volunteers
Yes
Inclusion criteria
* Adults ages 30-50 years * Non-smokers * Lean subjects with a BMI of 25 or less, gender specific normal body fat, * Obese, non-diabetic subjects with a BMI between 30 and 50 and taking no medications affecting glucose metabolism or lipid metabolism * subjects scheduled for an elective laparoscopic or laparotomy abdominal surgery
Exclusion criteria
* Receiving corticosteroid therapy or have a history of asthma, COPD or atopic syndrome * taking any biologic treatment for any autoimmune disorder * Taking any leukotriene receptor antagonist acting medications (ex. Montelukast) * smoking
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Number of eosinophils in adipose tissue | baseline | eosinophil number will be measured by flow cytometry in subcutaneous (superficial) adipose tissue |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Correlation between eosinophil content and body mass index (BMI) | baseline | Measured as a linear regression between BMI (defined as the weight in Kg/height in square m; kg/m2) and eosinophil number in the fat |
Other
| Measure | Time frame | Description |
|---|---|---|
| Quantification of the difference in gene methylation in eosinophils from lean and obese subjects | baseline | Gene methylation of eosinophils will be quantified in samples derived from lean and obese fat biopsy |
Countries
United States
Outcome results
None listed