Assessment of the Trophic and Anti-inflammatory Effect of SVF Added With HPL
Conditions
Brief summary
Osteoarthritis (OA) is a highly progressive and debilitating joint disease. Recently, it was demonstrated the existence of an important link between OA inflammation and progression of structural changes. Therapy with intra-articular injection of stromal vascular fraction (SVF) holds great promises and its efficacy could be further augmented with the addition of biological adjuvants. In the present project, we aim at demonstrating that human platelet lysate (HPL) increases the intrinsic beneficial properties of SVF on cartilage regeneration and joint environment. Moreover, we want to verify if SVF-conditioned medium (CM) obtained from SVF primed with HPL yields comparable or superior outcomes than whole SVF.
Interventions
OTHERisolation of waste material and culturing with different supplement culture media
isolation of waste material and culturing with different supplement culture media
Sponsors
I.R.C.C.S Ospedale Galeazzi-Sant'Ambrogio
Study design
Observational model
COHORT
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
18 Years to 75 Years
Healthy volunteers
Yes
Inclusion criteria
OA patients * Subscription of informed consent * BMI \< 30 * age between 55-75 years included * Kelgrenn-Lawrence equal or superior to grade III * presence of synovitis * patients undergoing knee replacement * suspension of NSAIDs from one week before the surgical procedure according to the standard clinical practice Healthy subjects from aesthetic plastic surgery * Subscription of informed consent * BMI \< 30 * age between 18-50 years included * subjects undergoing liposuction and/or abdominoplasty
Exclusion criteria
* HCV, HIV, HBV, TPHA infection * Pregnancy (auto declaration)
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Expression of trophic-related genes in cartilage samples treated with SVF added or not with HPL | surgery as scheduled by clinic routine | With this project, we expect to demonstrate that HPL increases the beneficial properties of SVF on cartilage regeneration and joint environment. We envision that HPL will increase expression of genes involved in ECM synthesis, thus enhancing cartilage regeneration. Based on previous published data, we will consider as statistical a difference between treated (SVF + HPL) and not treated samples (SVF) of two folds in expression of genes involved in ECM production (e.g. COL2A1, ACAN, SOX9). A fold increase equal to 2 is the minimum value to consider biologically relevant the influence of the treatment compared to a control group in which the gene expression is considered to be equal to 1. If our hypothesis will be confirmed, this will allow to improve the current therapy for OA with a biological adjuvant that reduces disease progression and helps cartilage regeneration. |
Contacts
Primary ContactSilvia Palombella, PhD
Outcome results
None listed