Kidney Cancer
Conditions
Keywords
Renal Cell Carcinoma, Expanded Genome-Wide Association
Brief summary
In the last decade, investigators from the Department of Cancer Epidemiology and Genetics (National Cancer Institute, USA) have conducted genome-wide association (GWAS) studies of renal cell carcinoma. Dr. Mark PURDUE and Dr. Stephen CAHNOCK (Department of Epidemiology of Cancer and Genetics, NCI) propose to expand their genome-wide association study (Expanded GWAS) by genotyping approximately 10,000 additional cases of kidney cancer patients, in collaboration with US institutions, South-American and European. This study describes the participation of the French Kidney Cancer Research Network (UroCCR) in the Expanded GWAS research, under the coordination of Professor BERNHARD (Bordeaux University Hospital).
Detailed description
The aim of the Expanded GWAS study is to better understand the role of common genetic variants in susceptibility to renal cell carcinoma (RCC). The participation of the French UroCCR network consists in completing the bio-collection of the UroCCR cohort by preserving constitutional DNA and to provide DNA samples to the US NCI team. This will increase the number of samples for meta-analysis and contribute to better identify clear cell kidney cancer susceptibility loci.
Interventions
PROCEDUREIntervention in the UroCCR sites
1. Blood sampling of a 5ml tube per patient. (for eligible patients recruited in the study, after signing the consent) 2. DNA extraction: * For the patients of Bordeaux University Hospital, 2 aliquots will be constituted: * 1 aliquot for Expanded GWAS, containing at least 2 μg of DNA, * 1 aliquot for the UroCCR biobank. * For patients from other UroCCR centers, only 1 aliquot will be constituted: * 1 aliquot for Expanded GWAS, containing at least 2 μg of DNA. 3. Transmission of the extracted DNA samples to the Biological Resource Center (CRB) of Bordeaux.
PROCEDUREIntervention in the CRB of Bordeaux University Hospital
1. Temporary storage of DNA samples for Expanded GWAS before shipping to the NCI. Storage of samples for the DNA biobank. 2. Overall packing of GWAS samples for shipment to the NCI. 3. Shipping to the NCI: Constitutive DNA samples must be sent to the NCI by May 1, 2020 for inclusion in the Expanded GWAS study.
PROCEDUREIntervention at the Methodology and Data Management Centre of Bordeaux University Hospital
Transfer of the data associated with the samples to the NCI.
PROCEDURESubsequent intervention at the NCI (for information)
1. Samples will be genotyped at the NCI Cancer Genomics Research Lab using the Illumina Global Screening Network. 2. GWAS combined meta-analysis, based, among others, on the results from the UroCCR samples.
Sponsors
National Cancer Institute (NCI)
University Hospital, Bordeaux
Study design
Observational model
CASE_ONLY
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
18 Years to 100 Years
Healthy volunteers
No
Inclusion criteria
* Patients over 18, * Confirmed cases of renal cell carcinoma (any RCC histology) of European or African origin, * Patients included in the UroCCR study, * Free, informed, written and signed consent by the participant and the investigator (at the latest on the day of inclusion and before any investigation required by the research) for specific blood collection for the purpose of DNA extraction, * Programmed or ongoing management for kidney tumor, * Constitutional DNA available (whole blood) for selected cases, * Affiliated person or beneficiary of a social security scheme.
Exclusion criteria
* Patients below 18, * Refusal of consent or participation.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Identification of new clear cell Renal Cell Carcinoma (ccRCC) risk loci | Inclusion Visit | From the DNA sample collected at inclusion visit, and the derived Single Nucleotide Polymorphisms (SNPs) genotype data: Association between evaluated genetic variants (SNPs) and renal cell carcinoma will be statistically determined if the p-value is found to be close to or lower than the Genome-wide significance threshold (p \<5\*10-8). |
Countries
France
Outcome results
None listed